Oncolytic alphavirus-induced extracellular vesicles counteract the immunosuppressive effect of melanoma-derived extracellular vesicles

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversity of Groningen-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorBhatt, Darshak K.-
Autor(es): dc.creatorBoerma, Annemarie-
Autor(es): dc.creatorBustos, Silvina Odete-
Autor(es): dc.creatorOtake, Andréia Hanada-
Autor(es): dc.creatorMurillo Carrasco, Alexis Germán-
Autor(es): dc.creatorReis, Patrícia Pintor-
Autor(es): dc.creatorChammas, Roger-
Autor(es): dc.creatorDaemen, Toos-
Autor(es): dc.creatorAndrade, Luciana Nogueira de Sousa-
Data de aceite: dc.date.accessioned2025-08-21T20:43:17Z-
Data de disponibilização: dc.date.available2025-08-21T20:43:17Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2025-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1038/s41598-024-82331-9-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/299265-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/299265-
Descrição: dc.descriptionExtracellular vesicles (EVs)-mediated communication by cancer cells contributes towards the pro-tumoral reprogramming of the tumor microenvironment. Viral infection has been observed to alter the biogenesis and cargo of EVs secreted from host cells in the context of infectious biology. However, the impact of oncolytic viruses on the cargo and function of EVs released by cancer cells remains unknown. Here we show that upon oncolytic virotherapy with Semliki Forest virus-based replicon particles (rSFV), metastatic melanoma cells release EVs with a distinct biochemical profile and do not lead to suppression of immune cells. Specifically, we demonstrate that viral infection causes a differential loading of regulatory microRNAs (miRNAs) in EVs in addition to changes in their physical features. EVs derived from cancer cells potentially suppress splenocyte proliferation and induce regulatory macrophages. In contrast, EVs obtained from rSFV-infected cells did not exhibit such effects. Our results thus show that rSFV infection induces changes in the immunomodulatory properties of melanoma EVs, which may contribute to enhancing the therapeutic efficacy of virotherapy. Finally, our results show that the use of an oncolytic virus capable of a single-round of infection allows the analysis of EVs secreted from infected cells while preventing interference from extracellular virus particles.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Medical Microbiology and Infection Prevention University Medical Center Groningen University of Groningen-
Descrição: dc.descriptionCenter for Translational Research in Oncology (LIM/24) Instituto do Cancer do Estado de Sao Paulo Hospital das Clinicas HCFMUSP Faculdade de Medicina Universidade de Sao Paulo-
Descrição: dc.descriptionComprehensive Center for Precision Oncology (C2PO) Universidade de Sao Paulo-
Descrição: dc.descriptionDepartment of Surgery and Orthopedics and Experimental Research Unity (UNIPEX) Faculdade de Medicina Universidade Estadual Paulista (UNESP)-
Descrição: dc.descriptionDepartment of Surgery and Orthopedics and Experimental Research Unity (UNIPEX) Faculdade de Medicina Universidade Estadual Paulista (UNESP)-
Descrição: dc.descriptionFAPESP: 2020/09176-8-
Idioma: dc.languageen-
Relação: dc.relationScientific Reports-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectExtracellular vesicles-
Palavras-chave: dc.subjectImmunomodulatory-
Palavras-chave: dc.subjectMelanoma-
Palavras-chave: dc.subjectOncolytic virus-
Título: dc.titleOncolytic alphavirus-induced extracellular vesicles counteract the immunosuppressive effect of melanoma-derived extracellular vesicles-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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