Dual-species biofilm of Streptococcus mutans and Candida albicans produces subsurface caries lesions on bovine enamel

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorSantana, Jéssica Silva-
Autor(es): dc.creatorDelbem, Alberto Carlos Botazzo-
Autor(es): dc.creatorPessan, Juliano Pelim-
Autor(es): dc.creatorSampaio, Caio-
Autor(es): dc.creatorde Morais, Leonardo Antônio-
Autor(es): dc.creatorPereira, Taynara Leandro-
Autor(es): dc.creatorMonteiro, Douglas Roberto-
Autor(es): dc.creatorHosida, Thayse Yumi-
Data de aceite: dc.date.accessioned2025-08-21T15:29:23Z-
Data de disponibilização: dc.date.available2025-08-21T15:29:23Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-10-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.archoralbio.2024.106029-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/299234-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/299234-
Descrição: dc.descriptionObjectives: To develop a protocol for forming subsurface caries lesions on bovine enamel by dual-species biofilms of Streptococcus mutans and Candida albicans in vitro. Design: Biofilms were grown on bovine enamel specimens in artificial saliva (AS) for seven days. After 24 h of formation, the AS was supplemented or not with fluoride (F) using sodium fluoride (0.005 or 0.008 ppm F), and the biofilms were exposed or not to a 20 % sucrose solution (reproducing a cariogenic challenge) once/day. On the seventh day, the biofilms were harvested and had their extracellular polysaccharides (EPS) and inorganic components analyzed. The specimens were subjected to computed X-ray microtomography analysis to determine their mineral concentration. Data were compared using two-way analyses of variance, followed by Fisher's LSD or Student–Newman–Keuls tests (p < 0.05). Results: Biofilms exposed to the cariogenic challenge had significantly higher EPS concentrations than those not exposed, regardless of the presence of F. For biofilms grown with 0.008 ppm F, those exposed to the cariogenic challenge had lower F levels than those not exposed. For biofilms exposed to the cariogenic challenge, those grown with 0.008 ppm F had lower lesion depths and integrated mineral loss, and higher outer layers than those grown without F. Conclusions: The dual biofilm model assessed was able to create subsurface caries lesions in bovine enamel in vitro, which was influenced by the presence of F in the culture medium and exposure to sucrose.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionDepartment of Preventive and Restorative Dentistry São Paulo State University (UNESP) School of Dentistry, Araçatuba-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery São Paulo State University (UNESP) School of Dentistry, Araçatuba-
Descrição: dc.descriptionDepartment of Preventive and Restorative Dentistry São Paulo State University (UNESP) School of Dentistry, Araçatuba-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery São Paulo State University (UNESP) School of Dentistry, Araçatuba-
Descrição: dc.descriptionCAPES: 001-
Idioma: dc.languageen-
Relação: dc.relationArchives of Oral Biology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBiofilms-
Palavras-chave: dc.subjectDemineralization-
Palavras-chave: dc.subjectDental caries-
Palavras-chave: dc.subjectDental enamel-
Título: dc.titleDual-species biofilm of Streptococcus mutans and Candida albicans produces subsurface caries lesions on bovine enamel-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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