Orexin mechanisms in the prelimbic cortex modulate the expression of contextual conditioned fear

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorBehavioural Neuroscience Institute (INeC)-
Autor(es): dc.contributorBoehringer Ingelheim Pharmaceuticals Die Gesellschaft mit Beschränkter Haftung & Compagnie Kommanditgesellschaft-
Autor(es): dc.creatorOliveira, Gabriela V. M.-
Autor(es): dc.creatorHernandes, Paloma M.-
Autor(es): dc.creatorSantos, Fábio H. dos-
Autor(es): dc.creatorSoares, Victor P. M. N.-
Autor(es): dc.creatorFalconi‑Sobrinho, Luiz Luciano-
Autor(es): dc.creatorCoimbra, Norberto C.-
Autor(es): dc.creatorWotjak, Carsten T.-
Autor(es): dc.creatorAlmada, Rafael Carvalho-
Data de aceite: dc.date.accessioned2025-08-21T17:27:32Z-
Data de disponibilização: dc.date.available2025-08-21T17:27:32Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2025-03-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s00213-024-06701-x-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/299213-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/299213-
Descrição: dc.descriptionRationale: Despite the existing anatomical and physiological evidence pointing to the involvement of orexinergic projections from the lateral hypothalamus (LH) in regulating fear-related responses, little is known regarding the contribution of the orexin system in the prelimbic cortex (PL) on contextual fear. Objectives: We investigated the role of orexin-A (OrxA) and orexin type 1 receptors (Orx1R) in the PL during the expression of contextual conditioned fear in mice. Methods: Neural tract tracing of the LH-PL pathway and Orx1R immunoreactivity in the PL of C57BL/6 male mice were performed. In a pharmacological approach, the animals were treated with either the Orx1R selective antagonist SB 334,867 (3, 30, and 300 nM/0.1 µL) or OrxA (28, 70, and 140 pmol/0.1 µL) in the PL before the test session of contextual fear conditioning. Results: Neural tract tracing deposits in the LH showed some perikarya, mainly axons and terminal buttons in the PL, suggesting LH-PL reciprocate pathways. Furthermore, we showed a profuse network comprised of Orx1R-labeled thin varicose fibers widely distributed in the same field of LH-PL pathways projection. The selective blockade of Orx1R with SB 334,867 at 30 and 300 nM in the PL caused a decrease in freezing response, whereas the treatment with OrxA at 140 pmol promoted an increase in freezing response. Conclusion: In summary, these data confirmed an anatomical link between LH and PL, established the presence of Orx1R in the PL, and a modulatory role of the orexin system in such structure, possibly mainly via Orx1R, during contextual fear conditioning.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionGerman-Israeli Foundation for Scientific Research and Development-
Descrição: dc.descriptionLaboratory of Neurobiology and Neurobiotechnology Department of Biological Sciences School of Sciences Humanities and Languages of the São Paulo State University (Unesp)-
Descrição: dc.descriptionLaboratory of Neuroanatomy and Neuropsychobiology Department of Pharmacology Ribeirão Preto Medical School of the University of São Paulo (FMRP-USP)-
Descrição: dc.descriptionBehavioural Neuroscience Institute (INeC)-
Descrição: dc.descriptionNAP-USP-Neurobiology of Emotions Research Centre (NuPNE) Ribeirão Preto Medical School of the University of São Paulo-
Descrição: dc.descriptionCentral Nervous System Diseases Research Boehringer Ingelheim Pharmaceuticals Die Gesellschaft mit Beschränkter Haftung & Compagnie Kommanditgesellschaft-
Descrição: dc.descriptionLaboratory of Neurobiology and Neurobiotechnology Department of Biological Sciences School of Sciences Humanities and Languages of the São Paulo State University (Unesp)-
Descrição: dc.descriptionFAPESP: 019/15757-6-
Descrição: dc.descriptionCAPES: 142030/2020–1-
Descrição: dc.descriptionFAPESP: 2018/03898-1-
Descrição: dc.descriptionFAPESP: 2019/01713-7-
Descrição: dc.descriptionFAPESP: 2019/05255–3-
Descrição: dc.descriptionFAPESP: 2020/02724-0-
Descrição: dc.descriptionFAPESP: 2020/15050-7-
Descrição: dc.descriptionFAPESP: 2021/06557-3-
Descrição: dc.descriptionFAPESP: 2021/14073-6-
Descrição: dc.descriptionCAPES: 88887.498215/2020-00-
Descrição: dc.descriptionGerman-Israeli Foundation for Scientific Research and Development: I-1442-421. 13/2017-
Formato: dc.format521-532-
Idioma: dc.languageen-
Relação: dc.relationPsychopharmacology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectContextual fear conditioning-
Palavras-chave: dc.subjectMedial prefrontal cortex-
Palavras-chave: dc.subjectOrexin type 1 receptor-
Palavras-chave: dc.subjectOrexin-A-
Palavras-chave: dc.subjectPrelimbic cortex-
Título: dc.titleOrexin mechanisms in the prelimbic cortex modulate the expression of contextual conditioned fear-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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