Landscape transcriptomic analysis of bovine follicular cells during key phases of ovarian follicular development

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual de Londrina (UEL)-
Autor(es): dc.contributorViçosa Federal University-
Autor(es): dc.contributorUniversity of Wisconsin-Madison-
Autor(es): dc.contributorUniversity of Calgary-
Autor(es): dc.creatorMogollón García, Henry David-
Autor(es): dc.creatorde Andrade Ferrazza, Rodrigo-
Autor(es): dc.creatorOchoa, Julian Camilo-
Autor(es): dc.creatorde Athayde, Flávia Florencio-
Autor(es): dc.creatorVidigal, Pedro Marcus Pereira-
Autor(es): dc.creatorWiltbank, Milo-
Autor(es): dc.creatorKastelic, John Patrick-
Autor(es): dc.creatorSartori, Roberto-
Autor(es): dc.creatorFerreira, João Carlos Pinheiro-
Data de aceite: dc.date.accessioned2025-08-21T19:59:19Z-
Data de disponibilização: dc.date.available2025-08-21T19:59:19Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1186/s40659-024-00558-2-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/299186-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/299186-
Descrição: dc.descriptionBackground: There are many gaps in our understanding of the mechanisms involved in ovarian follicular development in cattle, particularly regarding follicular deviation, acquisition of ovulatory capacity, and preovulatory changes. Molecular evaluations of ovarian follicular cells during follicular development in cattle, especially serial transcriptomic analyses across key growth phases, have not been reported. This study aims to address this gap by analyzing gene expression using RNA-seq in granulosa and antral cells recovered from ovarian follicular fluid during critical phases of ovarian follicular development in Holstein cows. Results: Integrated analysis of gene ontology (GO), gene set enrichment (GSEA), protein–protein interaction (PPI), and gene topology identified that differentially expressed genes (DEGs) in the largest ovarian follicles at deviation (Dev) were primarily involved in FSH-negative feedback, steroidogenesis, cell proliferation, apoptosis, and the prevention of early follicle rupture. In contrast, DEGs in the second largest follicles (DevF2) were mainly related to loss of cell viability, apoptosis, and immune cell invasion. In the dominant (PostDev) and preovulatory (PreOv) follicles, DEGs were associated with vascular changes and inflammatory responses. Conclusions: The transcriptome of ovarian follicular fluid cells had a predominance of granulosa cells in the dominant follicle at deviation, with upregulation of genes involved in cell viability, steroidogenesis, and apoptosis prevention, whereas in the non-selected follicle there was upregulation of cell death-related transcripts. Immune cell transcripts increased significantly after deviation, particularly in preovulatory follicles, indicating strong intrafollicular chemotactic activity. We inferred that immune cell invasion occurred despite an intact basal lamina, contributing to follicular maturation. Graphical Abstract: (Figure presented.)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science São Paulo State University (UNESP), Rua Prof. Doutor Walter Mauricio Correa, s/n, São Paulo-
Descrição: dc.descriptionDepartment of Genetic Evolution Microbiology and Immunology. Biology Institute Campinas State University, São Paulo-
Descrição: dc.descriptionComputational Systems Biology Laboratory (CSBL) Institut Pasteur University of São Paulo (USP)-
Descrição: dc.descriptionDepartment of Animal Science Londrina State University, Paraná-
Descrição: dc.descriptionDepartment of Animal Production and Health School of Veterinary Medicine São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionCenter of Biological Sciences Viçosa Federal University, Minas Gerais-
Descrição: dc.descriptionDepartment of Animal & Dairy Sciences University of Wisconsin-Madison-
Descrição: dc.descriptionFaculty of Veterinary Medicine University of Calgary-
Descrição: dc.descriptionDepartment of Animal Science Luiz de Queiroz College of Agriculture (ESALQ) University of São Paulo, São Paulo-
Descrição: dc.descriptionDepartment of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science São Paulo State University (UNESP), Rua Prof. Doutor Walter Mauricio Correa, s/n, São Paulo-
Descrição: dc.descriptionDepartment of Animal Production and Health School of Veterinary Medicine São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionCNPq: 190043/2017-2-
Descrição: dc.descriptionFAPESP: 2011/20449-7-
Descrição: dc.descriptionFAPESP: 2012/18297-7-
Idioma: dc.languageen-
Relação: dc.relationBiological Research-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectDeviation-
Palavras-chave: dc.subjectGenes-
Palavras-chave: dc.subjectHolstein cows-
Palavras-chave: dc.subjectSteroidogenesis-
Título: dc.titleLandscape transcriptomic analysis of bovine follicular cells during key phases of ovarian follicular development-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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