Toxicological assessment of niga-ichigoside F1 and 2β,3β-19α-trihydroxyursolic acid derived from Rubus imperialis (Rosaceae): determining the safety using human peripheral blood mononuclear cells (PBMC)

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorVale do Itajaí University (UNIVALI)-
Autor(es): dc.contributorCECAV-
Autor(es): dc.contributorUniversidade Estadual de Londrina (UEL)-
Autor(es): dc.creatorAlmeida-Terassi, Laíza Moura-
Autor(es): dc.creatorde Almeida, Laine Andreotti-
Autor(es): dc.creatorAlves, Greice Rafaele-
Autor(es): dc.creatorCechinel-Filho, Valdir-
Autor(es): dc.creatorNiero, Rivaldo-
Autor(es): dc.creatorde Mascarenhas Gaivão, Isabel O’Neill-
Autor(es): dc.creatorMantovani, Mário Sérgio-
Autor(es): dc.creatorMaistro, Edson Luis-
Data de aceite: dc.date.accessioned2025-08-21T23:44:56Z-
Data de disponibilização: dc.date.available2025-08-21T23:44:56Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1080/15287394.2025.2484350-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/299175-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/299175-
Descrição: dc.descriptionRubus imperialis Chum. Schl. (Rosaceae) is used in traditional medicine as a remedy to treat diabetes and other diseases. However, there are reports that the extract of this plant and others of the Rubus genus may produce cellular and/or genetic toxicity. Niga-ichigoside F1 (NIF1) and 2β,3β-19α-trihydroxyursolic acid (2B) are major compounds found in R. imperialis extract known to exert pharmacological effects which may be responsible for the observed toxicity. Thus, this study was aimed to examine the cytogenotoxic potential of NIF1 and 2B in human peripheral blood mononuclear cells (PBMC) following acute exposure. Cytotoxicity was determined by trypan blue staining and genotoxicity by micronucleus and comet assays. Data obtained showed that both compounds, at concentrations ranging from 0.1 and 20 µg/ml, did not markedly affect cell viability. In addition, genetic toxicity testing (comet assay) detected no evidence of DNA damage or chromosomal mutations by the micronucleus test. Under these experimental conditions and considering that both compounds exhibit biological effects at low concentrations, it may be concluded that NIF1 and 2B did not induce cytogenotoxic effects in primary human PBMC.-
Descrição: dc.descriptionFaculty of Philosophy and Sciences Speech and Hearing Therapy Department São Paulo State University (UNESP)-
Descrição: dc.descriptionPrograma de Pós-Graduação em Ciências Farmacêuticas e Núcleo de Investigações Químico-Farmacêuticas (NIQFAR) Vale do Itajaí University (UNIVALI)-
Descrição: dc.descriptionDepartment of Genetics and Biotechnology University of Trás–os–Montes and Alto Douro CECAV, Vila Real-
Descrição: dc.descriptionDepartment of General Biology Londrina State University (UEL)-
Descrição: dc.descriptionFaculty of Philosophy and Sciences Speech and Hearing Therapy Department São Paulo State University (UNESP)-
Idioma: dc.languageen-
Relação: dc.relationJournal of Toxicology and Environmental Health - Part A: Current Issues-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subject2β,3β-19α-trihydroxyursolic acid toxicity-
Palavras-chave: dc.subjectcomet assay-
Palavras-chave: dc.subjectmicronucleus test-
Palavras-chave: dc.subjectNiga-ichigoside F1 toxicity-
Palavras-chave: dc.subjectRosaceae toxicity-
Título: dc.titleToxicological assessment of niga-ichigoside F1 and 2β,3β-19α-trihydroxyursolic acid derived from Rubus imperialis (Rosaceae): determining the safety using human peripheral blood mononuclear cells (PBMC)-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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