Building the taxonomic profile of the Riniaie Marwah hot spring of Kishtwar in Jammu and Kashmir: the first high-throughput sequencing-based metagenome study

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Autor(es): dc.contributorLovely Profess Univ-
Autor(es): dc.contributorUniv Jammu-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorBrazil Univ-
Autor(es): dc.contributorIslamic Azad Univ-
Autor(es): dc.creatorWani, Atif Khurshid-
Autor(es): dc.creatorAhmad, Suhail-
Autor(es): dc.creatorAmerico-Pinheiro, Juliana Heloisa Pine-
Autor(es): dc.creatorTizro, Nazli-
Autor(es): dc.creatorSingh, Reena-
Data de aceite: dc.date.accessioned2025-08-21T21:19:08Z-
Data de disponibilização: dc.date.available2025-08-21T21:19:08Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-11-30-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/299168-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/299168-
Descrição: dc.descriptionBackground and Objectives: Rinaie Marwah hot spring Kishtwar (RMHSK) is one of the geothermal springs located at 33 degrees 51 ' 51 '' N 75 degrees 32 ' 07 '' E with an elevation of 2134 meters above sea level in Jammu and Kashmir, India. We aimed to study the microbial diversity of this geothermal spring using metagenomics.Materials and Methods: In the present study, physiochemical parameters including temperature (65-75oC), pH (6. 9-8. 8), hardness (250 ppm), and mineral content was measured along with the microbial diversity using Illumina MiSeq metage-nome-based 16s amplicon sequencing (V3-V4). The sequence reads were classified taxonomically into 31 phyla, 71 classes, 152 orders, 256 families, 410 genus, and 665 species. QIIME 2 (Quantitative Insights into Microbial Ecology), an extensible, powerful, and decentralized analytical tool, was used for taxonomic analysis.Results: Bacteroidota (32. 57%) was the dominant phylum, Bacteroidia (32. 51%) the dominant class, Bacteroidales (16. 6%) the dominant order, and Lentimicrobiaceae (14. 23%) was the dominant family per the abundance analysis. Shannon (2. 28) and Chao 1 (87. 0) diversity indices support the existence of higher microbial diversity in RMHSK (50717 OTUs).Conclusion: The microbial diversity of RMHSK is reported for the first time through a metagenomic study. Identification of microorganisms with characteristics that are relevant to industries.-
Descrição: dc.descriptionLovely Profess Univ, Sch Bioengn & Biosci, Dept Biotechnol, Jalandhar, Punjab, India-
Descrição: dc.descriptionUniv Jammu, Dept Remote Sensing & GIS, Jammu, India-
Descrição: dc.descriptionSao Paulo State Univ UNESP, Sch Agron Sci, Dept Forest Sci Soils & Environm, Botucatu, Brazil-
Descrição: dc.descriptionBrazil Univ, Dept Environm Sci, Grad Program Environm Sci, Sao Paulo, Brazil-
Descrição: dc.descriptionIslamic Azad Univ, Coll Nat Resources & Environm, Dept Environm, Sci & Res Branch, Tehran, Iran-
Descrição: dc.descriptionSao Paulo State Univ UNESP, Sch Agron Sci, Dept Forest Sci Soils & Environm, Botucatu, Brazil-
Formato: dc.format723-733-
Idioma: dc.languageen-
Publicador: dc.publisherUniv Medical Sciences-danishgah-i Ulum-i Pizishki-i Tihran-
Relação: dc.relationIranian Journal Of Microbiology-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectHot spring-
Palavras-chave: dc.subjectMetagenome-
Palavras-chave: dc.subjectTaxonomy-
Palavras-chave: dc.subjectHigh throughput DNA sequencing-
Palavras-chave: dc.subjectIllumina sequencing-
Título: dc.titleBuilding the taxonomic profile of the Riniaie Marwah hot spring of Kishtwar in Jammu and Kashmir: the first high-throughput sequencing-based metagenome study-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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