Comparison of Three Serologic Tests for the Detection of Anti-Coxiella burnetii Antibodies in Patients with Q Fever

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorOtávio Magalhães Institute-
Autor(es): dc.contributorAdolfo Lutz Institute-
Autor(es): dc.creatorFrança, Danilo Alves de-
Autor(es): dc.creatorMioni, Mateus de Souza Ribeiro-
Autor(es): dc.creatorFornazari, Felipe-
Autor(es): dc.creatorRodrigues, Nássarah Jabur Lot-
Autor(es): dc.creatorPolido, Lucas Roberto Ferreira-
Autor(es): dc.creatorAppolinario, Camila Michele-
Autor(es): dc.creatorRibeiro, Bruna Letícia Devidé-
Autor(es): dc.creatorDuré, Ana Íris de Lima-
Autor(es): dc.creatorSilva, Marcos Vinicius Ferreira-
Autor(es): dc.creatorRichini-Pereira, Virgínia Bodelão-
Autor(es): dc.creatorLangoni, Helio-
Autor(es): dc.creatorMegid, Jane-
Data de aceite: dc.date.accessioned2025-08-21T20:34:57Z-
Data de disponibilização: dc.date.available2025-08-21T20:34:57Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-07-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/pathogens12070873-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/299144-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/299144-
Descrição: dc.descriptionThe performance of a commercial immunofluorescence assay (IFA commercial), an in-house immunofluorescence assay (IFA in-house) and an indirect enzyme-linked immunosorbent assay (ELISA) were evaluated in the detection of antibodies anti-C. burnetii in the serum of Q fever patients and persons without the disease. For the study, seropositive and seronegative samples for Q fever (n = 200) from a serum bank of the Instituto Adolfo Lutz in Brazil were used. Commercial IFA was considered in this study as the gold standard for diagnosing Q fever. The in-house IFA demonstrated good agreement with the commercial test, showing high sensitivity (91%) and specificity (97%) compared to the gold standard, with a Kappa coefficient of 0.8954. The indirect ELISA test showed lower agreement with the gold standard, showing low sensitivity (67%), although the specificity of the technique was high (97%) and the Kappa coefficient was moderate (0.6631). In-house IFA is an excellent alternative for diagnosing Q fever.-
Descrição: dc.descriptionDepartment of Veterinary Hygiene and Public Health São Paulo State University-
Descrição: dc.descriptionBotucatu Medical School São Paulo State University-
Descrição: dc.descriptionEzequiel Dias Foundation Otávio Magalhães Institute-
Descrição: dc.descriptionRegional Laboratories Center II Adolfo Lutz Institute-
Descrição: dc.descriptionDepartment of Veterinary Hygiene and Public Health São Paulo State University-
Descrição: dc.descriptionBotucatu Medical School São Paulo State University-
Idioma: dc.languageen-
Relação: dc.relationPathogens-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectacute disease-
Palavras-chave: dc.subjectELISA-
Palavras-chave: dc.subjecthumans-
Palavras-chave: dc.subjectimmunofluorescence-
Palavras-chave: dc.subjectQ fever-
Palavras-chave: dc.subjectserodiagnosis-
Título: dc.titleComparison of Three Serologic Tests for the Detection of Anti-Coxiella burnetii Antibodies in Patients with Q Fever-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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