Titanium micro-nano textured surface with strontium incorporation improves osseointegration: an in vivo and in vitro study

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorExtremus Smart Surface-
Autor(es): dc.contributorEscuela de Odontología-
Autor(es): dc.contributorCenter of the Johannes Gutenberg University-
Autor(es): dc.creatorda COSTA FILHO, Pio Moerbeck-
Autor(es): dc.creatorMARCANTONIO, Camila Chiérici-
Autor(es): dc.creatorde OLIVEIRA, Diego Pedreira-
Autor(es): dc.creatorLOPES, Maria Eduarda Scordamaia-
Autor(es): dc.creatorPUETATE, Julio Cesar Sanchez-
Autor(es): dc.creatorFARIA, Luan Viana-
Autor(es): dc.creatorde CARVALHO, Letícia Freitas-
Autor(es): dc.creatorde MOLON, Rafael Scaf-
Autor(es): dc.creatorJUNIOR, Idelmo Rangel GARCIA-
Autor(es): dc.creatorNOGUEIRA, Andressa Vilas Bôas-
Autor(es): dc.creatorDESCHNER, James-
Autor(es): dc.creatorCIRELLI, Joni Augusto-
Data de aceite: dc.date.accessioned2025-08-21T21:40:17Z-
Data de disponibilização: dc.date.available2025-08-21T21:40:17Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1590/1678-7757-2024-0144-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/299113-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/299113-
Descrição: dc.descriptionObjectives: This study aimed to investigate the osseointegration of titanium (Ti) implants with micro-nano textured surfaces functionalized with strontium additions (Sr) in a pre-clinical rat tibia model. Methodology: Ti commercially pure (cp-Ti) implants were installed bilaterally in the tibia of 64 Holtzman rats, divided into four experimental groups (n=16/group): (1) Machined surface - control (C); (2) Micro-nano textured surface treatment (MN); (3) Micro-nano textured surface with Sr2+ addition (MNSr); and (4) Micro-nano textured surface with a higher complementary addition of Sr2+ (MNSr+). In total, two experimental euthanasia periods were assessed at 15 and 45 days (n=8/period). The tibia was subjected to micro-computed tomography (μ-CT), histomorphometry with the EXAKT system, removal torque (TR) testing, and gene expression analysis by PCR-Array of 84 osteogenic markers. Gene expression and protein production of bone markers were performed in an in vitro model with MC3T3-E1 cells. The surface characteristics of the implants were evaluated by scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDS), and laser scanning confocal microscopy. Results: SEM, confocal, and EDS analyses demonstrated the formation of uniform micro-nano textured surfaces in the MN group and Sr addition in the MNSr and MNSr+ groups. TR test indicated greater osseointegration in the 45- day period for treated surfaces. Histological analysis highlighted the benefits of the treatments, especially in cortical bone, in which an increase in bone-implant contact was found in groups MN (15 days) and MNSr (45 days) compared to the control group. Gene expression analysis of osteogenic activity markers showed modulation of various osteogenesis-related genes. According to the in vitro model, RT-qPCR and ELISA demonstrated that the treatments favored gene expression and production of osteoblastic differentiation markers. Conclusions: Micro-nano textured surface and Sr addition can effectively improve and accelerate implant osseointegration and is, therefore, an attractive approach to modifying titanium implant surfaces with significant potential in clinical practice.-
Descrição: dc.descriptionUniversidade Estadual Paulista - UNESP Faculdade de Odontologia de Araraquara Departamento de Diagnóstico e Cirurgia, Araraquara-
Descrição: dc.descriptionExtremus Smart Surface, São Carlos-
Descrição: dc.descriptionUniversidad San Francisco de Quito USFQ Escuela de Odontología Departmento de Periodoncia, Quito-
Descrição: dc.descriptionUniversidade Estadual Paulista - UNESP Faculdade de Odontologia de Araraçatuba Departamento de Diagnóstico e Cirurgia, SP-
Descrição: dc.descriptionUniversity Medical Mainz Center of the Johannes Gutenberg University Department of Periodontology and Operative Dentistry-
Descrição: dc.descriptionUniversidade Estadual Paulista - UNESP Faculdade de Odontologia de Araraquara Departamento de Diagnóstico e Cirurgia, Araraquara-
Descrição: dc.descriptionUniversidade Estadual Paulista - UNESP Faculdade de Odontologia de Araraçatuba Departamento de Diagnóstico e Cirurgia, SP-
Idioma: dc.languageen-
Relação: dc.relationJournal of Applied Oral Science-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAnimal model-
Palavras-chave: dc.subjectDental implant-
Palavras-chave: dc.subjectOsseointegration-
Palavras-chave: dc.subjectStrontium-
Palavras-chave: dc.subjectSurface modification-
Palavras-chave: dc.subjectTitanium implant-
Título: dc.titleTitanium micro-nano textured surface with strontium incorporation improves osseointegration: an in vivo and in vitro study-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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