Development of Ni-ZnO-ACE-2 peptide hybrids as electrochemical devices for SARS-CoV-2 spike protein detection

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Federal do ABC (UFABC)-
Autor(es): dc.contributorLeibniz-Institut für Polymerforschung Dresden e.V.-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorNunez, Freddy.A.-
Autor(es): dc.creatorSilva, Marcos R. de A.-
Autor(es): dc.creatorCilli, Eduardo M.-
Autor(es): dc.creatorBrandão, Sarah T.R.-
Autor(es): dc.creatorMüller, Martin-
Autor(es): dc.creatorFischer, Dieter-
Autor(es): dc.creatorBesford, Quinn A.-
Autor(es): dc.creatorAlves, Wendel A.-
Data de aceite: dc.date.accessioned2025-08-21T21:31:13Z-
Data de disponibilização: dc.date.available2025-08-21T21:31:13Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2025-06-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.bioelechem.2025.108899-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/299090-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/299090-
Descrição: dc.descriptionOwing to fast SARS-CoV-2 mutations, biosensors employing antibodies as biorecognition elements have presented problems with sensitivity and accuracy. To face these challenges, antibodies can be replaced with the human angiotensin converting enzyme 2 (ACE-2), where it has been shown that the affinity between ACE-2 and the receptor binding domain (RBD) increases with the emergence of new variants. Herein, we report on Ni-doped ZnO nanorod electrochemical biosensors employing an ACE-2 peptide (IEEQAKTFLDKFNHEAEDLFYQS-NH2) as a biorecognition element for detecting Spike (S) Wild-Type (WT) protein. The electrode was fully characterized in terms of electrochemical and physical properties. The sensor showed high cross reactivity with Spike protein B.1.1.7 and Spike protein B.1.351. Still, there was no cross reactivity with the Nucleocapsid protein WT, showing that the biosensor can identify ancestral WT S protein and S protein variants of concern. The device exhibited a LOD of 60.13 ng mL−1 across an S protein WT concentration range from 200 ng mL−1 to 1000 ng mL−1 and a LOQ of 182.22 ng mL−1. The calculated sensitivity and specificity were 88.88 and 100 %, respectively. These results proved that the Ni-ZnO sensor has promising prospects for SARS-CoV-2 detection and diagnosis of other viruses, employing peptides as biorecognition elements.-
Descrição: dc.descriptionCentro de Ciências Naturais e Humanas Universidade Federal do ABC, Santo André-
Descrição: dc.descriptionLeibniz-Institut für Polymerforschung Dresden e.V., Hohe Str. 6-
Descrição: dc.descriptionUniversidade Estadual Paulista (UNESP) Institute of Chemistry, Araraquara-
Descrição: dc.descriptionUniversidade Estadual Paulista (UNESP) Institute of Chemistry, Araraquara-
Idioma: dc.languageen-
Relação: dc.relationBioelectrochemistry-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectACE-2 peptide-
Palavras-chave: dc.subjectCOVID-19-
Palavras-chave: dc.subjectElectrochemical biosensor-
Palavras-chave: dc.subjectNi-ZnO nanorods-
Palavras-chave: dc.subjectSARS-CoV-2-
Palavras-chave: dc.subjectSARS-CoV-2 variants-
Título: dc.titleDevelopment of Ni-ZnO-ACE-2 peptide hybrids as electrochemical devices for SARS-CoV-2 spike protein detection-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.