Comparative Analysis of CAZymes from Trichoderma longibrachiatum LMBC 172 Cultured with Three Different Carbon Sources: Sugarcane Bagasse, Tamarind Seeds, and Hemicellulose Simulation

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Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorOklahoma State University-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorContato, Alex Graça-
Autor(es): dc.creatorBorelli, Tiago Cabral-
Autor(es): dc.creatorde Carvalho, Ana Karine Furtado-
Autor(es): dc.creatorBento, Heitor Buzetti Simões-
Autor(es): dc.creatorBuckeridge, Marcos Silveira-
Autor(es): dc.creatorRogers, Janet-
Autor(es): dc.creatorHartson, Steven-
Autor(es): dc.creatorPrade, Rolf Alexander-
Autor(es): dc.creatorPolizeli, Maria de Lourdes Teixeira de Moraes-
Data de aceite: dc.date.accessioned2025-08-21T19:10:25Z-
Data de disponibilização: dc.date.available2025-08-21T19:10:25Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-09-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/cleantechnol6030050-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/298986-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/298986-
Descrição: dc.descriptionThe examination of fungal secretomes has garnered attention for its potential to unveil the repertoire of secreted proteins, notably CAZymes (Carbohydrate-Active enzymes), across various microorganisms. This study presents findings on categorizing the secretome profile of CAZymes by their function and family, derived from the filamentous fungus Trichoderma longibrachiatum LMBC 172. The cultivation was performed through submerged fermentation with three distinct carbon sources: sugarcane bagasse, tamarind seeds, and a control simulating hemicellulose containing 0.5% beechwood xylan plus 0.5% oat spelt xylan. The secretome analysis revealed 206 distinct CAZymes. Each carbon source showed particularities and differences. Of these, 89 proteins were produced simultaneously with all the carbon sources; specifically, 41 proteins using only the hemicellulose simulation, 29 proteins when sugarcane bagasse was used as a carbon source, and only 3 when tamarind seeds were used. However, in this last condition, there was a high intensity of xyloglucanase GH74 production, thus reaffirming the richness of xyloglucan in the constitution of these seeds. When evaluating the proteins found in two conditions, 18 proteins were shown between the simulation of hemicellulose and sugarcane bagasse, 11 proteins between the simulation of hemicellulose and tamarind seeds, and 15 proteins between sugarcane bagasse and tamarind seeds. Among the proteins found, there are representatives of different families such as glycosyl hydrolases (GHs) that cleave cellulose, hemicellulose, pectin, or other components; carbohydrate esterases (CEs); polysaccharide lyases (PLs); carbohydrate-binding modules (CBMs); and auxiliary activity enzymes (AAs). These results demonstrate the importance of analyzing CAZymes secreted by microorganisms under different culture conditions.-
Descrição: dc.descriptionDepartment of Biochemistry and Immunology Faculty of Medicine of Ribeirão Preto University of São Paulo, Ribeirão Preto, SP-
Descrição: dc.descriptionDepartment of Microbiology and Molecular Genetics Oklahoma State University-
Descrição: dc.descriptionDepartment of Cellular and Molecular Biology and Pathogenic Bioagents Faculty of Medicine of Ribeirão Preto University of São Paulo, Ribeirão Preto, SP-
Descrição: dc.descriptionDepartment of Basic and Environmental Sciences Lorena School of Engineering University of São Paulo, SP-
Descrição: dc.descriptionDepartment of Bioprocess Engineering and Biotechnology School of Pharmaceutical Sciences São Paulo State University, SP-
Descrição: dc.descriptionDepartment of Botany Institute of Biosciences University of São Paulo, São Paulo, SP-
Descrição: dc.descriptionDepartment of Biochemistry and Molecular Biology Oklahoma State University-
Descrição: dc.descriptionDepartment of Biology Faculty of Philosophy Sciences and Letters of Ribeirão Preto University of São Paulo, Ribeirão Preto, SP-
Descrição: dc.descriptionDepartment of Bioprocess Engineering and Biotechnology School of Pharmaceutical Sciences São Paulo State University, SP-
Formato: dc.format994-1010-
Idioma: dc.languageen-
Relação: dc.relationClean Technologies-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCAZymes-
Palavras-chave: dc.subjectsecretome-
Palavras-chave: dc.subjectsugarcane bagasse-
Palavras-chave: dc.subjecttamarind seeds-
Palavras-chave: dc.subjectTrichoderma longibrachiatum-
Título: dc.titleComparative Analysis of CAZymes from Trichoderma longibrachiatum LMBC 172 Cultured with Three Different Carbon Sources: Sugarcane Bagasse, Tamarind Seeds, and Hemicellulose Simulation-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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