Mannose-Binding Lectins as Potent Antivirals against SARS-CoV-2

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Autor(es): dc.contributorUniversidade Federal de Uberlândia (UFU)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorFederal University of Cariri (UFCA)-
Autor(es): dc.contributorCEUMA University-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorBi-Institutional Platform for Translational Medicine-
Autor(es): dc.contributorState University of Southwest of Bahia-
Autor(es): dc.creatorGrosche, Victória Riquena-
Autor(es): dc.creatorSouza, Leandro Peixoto Ferreira-
Autor(es): dc.creatorFerreira, Giulia Magalhães-
Autor(es): dc.creatorGuevara-Vega, Marco-
Autor(es): dc.creatorCarvalho, Tamara-
Autor(es): dc.creatorSilva, Romério Rodrigues dos Santos-
Autor(es): dc.creatorBatista, Karla Lilian Rodrigues-
Autor(es): dc.creatorAbuna, Rodrigo Paolo Flores-
Autor(es): dc.creatorSilva, João Santana-
Autor(es): dc.creatorCalmon, Marília de Freitas-
Autor(es): dc.creatorRahal, Paula-
Autor(es): dc.creatorda Silva, Luis Cláudio Nascimento-
Autor(es): dc.creatorAndrade, Bruno Silva-
Autor(es): dc.creatorTeixeira, Claudener Souza-
Autor(es): dc.creatorSabino-Silva, Robinson-
Autor(es): dc.creatorJardim, Ana Carolina Gomes-
Data de aceite: dc.date.accessioned2025-08-21T22:51:30Z-
Data de disponibilização: dc.date.available2025-08-21T22:51:30Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-09-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/v15091886-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/298819-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/298819-
Descrição: dc.descriptionThe SARS-CoV-2 entry into host cells is mainly mediated by the interactions between the viral spike protein (S) and the ACE-2 cell receptor, which are highly glycosylated. Therefore, carbohydrate binding agents may represent potential candidates to abrogate virus infection. Here, we evaluated the in vitro anti-SARS-CoV-2 activity of two mannose-binding lectins isolated from the Brazilian plants Canavalia brasiliensis and Dioclea violacea (ConBR and DVL). These lectins inhibited SARS-CoV-2 Wuhan-Hu-1 strain and variants Gamma and Omicron infections, with selectivity indexes (SI) of 7, 1.7, and 6.5, respectively for ConBR; and 25, 16.8, and 22.3, for DVL. ConBR and DVL inhibited over 95% of the early stages of the viral infection, with strong virucidal effect, and also protected cells from infection and presented post-entry inhibition. The presence of mannose resulted in the complete lack of anti-SARS-CoV-2 activity by ConBR and DVL, recovering virus titers. ATR-FTIR, molecular docking, and dynamic simulation between SARS-CoV-2 S and either lectins indicated molecular interactions with predicted binding energies of −85.4 and −72.0 Kcal/Mol, respectively. Our findings show that ConBR and DVL lectins possess strong activities against SARS-CoV-2, potentially by interacting with glycans and blocking virus entry into cells, representing potential candidates for the development of novel antiviral drugs.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionLaboratory of Antiviral Research Institute of Biomedical Science (ICBIM) Federal University of Uberlândia (UFU)-
Descrição: dc.descriptionInstitute of Biosciences Languages and Exact Sciences (Ibilce) São Paulo State University (Unesp)-
Descrição: dc.descriptionInnovation Center in Salivary Diagnostic and Nanobiotechnology Institute of Biomedical Science (ICBIM) Federal University of Uberlândia (UFU)-
Descrição: dc.descriptionCenter of Agrarian Science and Biodiversity Federal University of Cariri (UFCA)-
Descrição: dc.descriptionLaboratory of Microbial Pathogenesis CEUMA University-
Descrição: dc.descriptionDepartment of Biochemistry and Immunology Ribeirão Preto Medical School University of São Paulo-
Descrição: dc.descriptionOswaldo Cruz Foundation (Fiocruz) Bi-Institutional Platform for Translational Medicine-
Descrição: dc.descriptionLaboratory of Bioinformatics and Computational Chemistry State University of Southwest of Bahia-
Descrição: dc.descriptionInstitute of Biosciences Languages and Exact Sciences (Ibilce) São Paulo State University (Unesp)-
Descrição: dc.descriptionCNPq: 307207/2021-8-
Descrição: dc.descriptionCNPq: 308474/2021-6-
Descrição: dc.descriptionCAPES: 88881.506794/2020-01-
Descrição: dc.descriptionCAPES: 88887.505971/2020-00-
Idioma: dc.languageen-
Relação: dc.relationViruses-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectantivirals-
Palavras-chave: dc.subjectCOVID-19-
Palavras-chave: dc.subjectglycans-
Palavras-chave: dc.subjectmannose-biding lectins-
Palavras-chave: dc.subjectnatural compounds-
Palavras-chave: dc.subjectSARS-CoV-2-
Título: dc.titleMannose-Binding Lectins as Potent Antivirals against SARS-CoV-2-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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