Uncovering Metabolic Alterations in HCT-116 Colon Cancer Cells upon Exposure to Bamboo Leaf Extract Obtained from Guadua incana Londoño

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Autor(es): dc.contributorPontificia Universidad Javeriana-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidad de los Andes-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorFederal University of Espírito Santo (UFES)-
Autor(es): dc.contributorUniversidad Nacional de Colombia-
Autor(es): dc.creatorChitiva, Luis Carlos-
Autor(es): dc.creatorSantamaría-Torres, Mary Andrea-
Autor(es): dc.creatorRezende-Teixeira, Paula-
Autor(es): dc.creatorBorlot, Jessica Rodrigues Pereira de Oliveira-
Autor(es): dc.creatorRomagna, Rodrigo de Almeida-
Autor(es): dc.creatorLondoño, Ximena-
Autor(es): dc.creatorKitagawa, Rodrigo Rezende-
Autor(es): dc.creatorCosta-Lotufo, Leticia V.-
Autor(es): dc.creatorPrieto-Rodríguez, Juliet A.-
Autor(es): dc.creatorCastro-Gamboa, Ian-
Autor(es): dc.creatorCosta, Geison Modesti-
Data de aceite: dc.date.accessioned2025-08-21T23:15:44Z-
Data de disponibilização: dc.date.available2025-08-21T23:15:44Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-07-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/molecules29132985-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/298750-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/298750-
Descrição: dc.descriptionMetabolic alterations are increasingly recognized as important aspects of colorectal cancer (CRC), offering potential avenues for identifying therapeutic targets. Previous studies have demonstrated the cytotoxic potential of bamboo leaf extract obtained from Guadua incana (BLEGI) against HCT-116 colon cancer cells. However, the altered metabolic pathways in these tumor cells remain unknown. Therefore, this study aimed to employ an untargeted metabolomic approach to reveal the metabolic alterations of the endometabolome and exometabolome of HCT-116 cells upon exposure to BLEGI treatment. First, a chemical characterization of the BLEGI was conducted through liquid chromatography coupled with mass spectrometry (LC-MS). Next, we assessed cell viability via MTT and morphological analysis using an immunofluorescence assay against colon cancer cells, and anti-inflammatory activity using an LPS-stimulated macrophage model. Subsequently, we employed LC-MS and proton nuclear magnetic resonance (1H-NMR) to investigate intra- and extracellular changes. Chemical characterization primarily revealed the presence of compounds with a flavone glycoside scaffold. Immunofluorescence analysis showed condensed chromatin and subsequent formation of apoptotic bodies, suggesting cell death by apoptosis. The results of the metabolomic analysis showed 98 differential metabolites, involved in glutathione, tricarboxylic acid cycle, and lipoic acid metabolism, among others. Additionally, BLEGI demonstrated significant nitric oxide (NO) inhibitory capacity in macrophage cells. This study enhances our understanding of BLEGI’s possible mechanism of action and provides fresh insights into therapeutic targets for treating this disease.-
Descrição: dc.descriptionGrupo de Investigación Fitoquímica Universidad Javeriana (GIFUJ) Department of Chemistry Faculty of Sciences Pontificia Universidad Javeriana-
Descrição: dc.descriptionNúcleo de Bioensaios Biossíntese e Ecofisiologia de Produtos Naturais (NuBBE) Institute of Chemistry São Paulo State University (UNESP)-
Descrição: dc.descriptionUniversidad de los Andes-
Descrição: dc.descriptionLaboratório de Farmacologia de Produtos Naturais Marinhos Institute of Biomedical Sciences University of São Paulo-
Descrição: dc.descriptionLaboratório de Triagem Biológica de Produtos Naturais Department of Pharmaceutical Sciences Federal University of Espírito Santo (UFES)-
Descrição: dc.descriptionFaculty of Agricultural Sciences Universidad Nacional de Colombia-
Descrição: dc.descriptionNúcleo de Bioensaios Biossíntese e Ecofisiologia de Produtos Naturais (NuBBE) Institute of Chemistry São Paulo State University (UNESP)-
Idioma: dc.languageen-
Relação: dc.relationMolecules-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectanti-inflammatory activity-
Palavras-chave: dc.subjectbamboo leaf extract-
Palavras-chave: dc.subjectcytotoxic activity-
Palavras-chave: dc.subjectendometabolome-
Palavras-chave: dc.subjectexometabolome-
Palavras-chave: dc.subjectGuadua incana-
Palavras-chave: dc.subjectHCT-116 cells-
Palavras-chave: dc.subjectmetabolomics-
Título: dc.titleUncovering Metabolic Alterations in HCT-116 Colon Cancer Cells upon Exposure to Bamboo Leaf Extract Obtained from Guadua incana Londoño-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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