Bone regeneration: The influence of composite HA/TCP scaffolds and electrical stimulation on TGF/BMP and RANK/RANKL/OPG pathways

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorFHO-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorNanyang Technological University-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorHelaehil, Júlia Venturini-
Autor(es): dc.creatorHuang, Boyang-
Autor(es): dc.creatorBartolo, Paulo-
Autor(es): dc.creatorSantamaria-JR, Milton-
Autor(es): dc.creatorCaetano, Guilherme Ferreira-
Data de aceite: dc.date.accessioned2025-08-21T22:06:46Z-
Data de disponibilização: dc.date.available2025-08-21T22:06:46Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2025-01-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.injury.2025.112158-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/298625-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/298625-
Descrição: dc.descriptionThe repair of critical-sized bone defects represents significant clinical challenge. An alternative approach is the use of 3D composite scaffolds to support bone regeneration. Hydroxyapatite (HA) and tri-calcium phosphate (β-TCP), combined with polycaprolactone (PCL), offer promising mechanical resistance and biocompatibility. Bioelectrical stimulation (ES) at physiological levels is proposed to reestablishes tissue bioeletrocity and modulates cell signaling communication, such as the BMP/TGF-β and the RANK/RANK-L/OPG pathways. This study aimed to evaluate the use HA/TCP scaffolds and ES therapy for bone regeneration and their impact on the TGF-β/BMP pathway, alongside their relationship with the RANK/RANKL/OPG pathway in critical bone defects. The scaffolds were implanted at the bone defect in animal model (calvarial bone) and the area was subjected to ES application twice a week at 10 µA intensity of current for 5 min each session. Samples were collected for histomorphometry, immunohistochemistry, and molecular analysis. The TGF-β/BMP pathway study showed the HA/TCP+ES group increased BMP-7 gene expression at 30 and 60 days, and also greater endothelial vascular formation. Moreover, the HA/TCP and HA/TCP+ES groups exhibited a bone remodeling profile, indicated by RANKL/OPG ratio. HA/TCP scaffolds with ES enhanced vascular formation and mineralization initially, while modulation of the BMP/TGF pathway maintained bone homeostasis, controlling resorption via ES with HA/TCP.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionUniversity Center of Hermínio Ometto Foundation FHO, Araras 13607-339, SP-
Descrição: dc.descriptionDivision of Dermatology Department of Internal Medicine Ribeirao Preto Medical School University of Sao Paulo, 05508-060-
Descrição: dc.descriptionSingapore Centre for 3D Printing School of Mechanical and Aerospace Engineering Nanyang Technological University-
Descrição: dc.descriptionGraduate Program of Orthodontics University Center of Hermínio Ometto Foundation FHO, Araras 13607-339, SP-
Descrição: dc.descriptionDepartment of Social and Pediatric Dentistry Institute of Science and Technology São Paulo State University - Unesp, São José dos Campos, 12245-000-
Descrição: dc.descriptionDepartment of Social and Pediatric Dentistry Institute of Science and Technology São Paulo State University - Unesp, São José dos Campos, 12245-000-
Descrição: dc.descriptionFAPESP: 2018/21167–4-
Descrição: dc.descriptionCNPq: 423710/2018-4-
Idioma: dc.languageen-
Relação: dc.relationInjury-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBioprinting-
Palavras-chave: dc.subjectElectrical stimulation-
Palavras-chave: dc.subjectMicrocurrent-
Palavras-chave: dc.subjectTGF pathway-
Palavras-chave: dc.subjectTissue engineering-
Título: dc.titleBone regeneration: The influence of composite HA/TCP scaffolds and electrical stimulation on TGF/BMP and RANK/RANKL/OPG pathways-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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