Biochemical and hemostatic description of a thrombin-like enzyme TLBro from Bothrops roedingeri snake venom

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorUniversidad Tecnológica del Perú-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidad Católica de Santa María-
Autor(es): dc.contributorUniversidad Nacional de San Agustín de Arequipa-
Autor(es): dc.contributorUniversidad San Ignacio de Loyola-
Autor(es): dc.contributorUniversidad Peruana de Ciencias Aplicadas-
Autor(es): dc.creatorVilca-Quispe, Augusto-
Autor(es): dc.creatorAlvarez-Risco, Aldo-
Autor(es): dc.creatorGomes Heleno, Mauricio Aurelio-
Autor(es): dc.creatorPonce-Fuentes, Emilio Alberto-
Autor(es): dc.creatorVera-Gonzales, Corina-
Autor(es): dc.creatorZegarra-Aragon, Herly Fredy Enrique-
Autor(es): dc.creatorAquino-Puma, Juan Luis-
Autor(es): dc.creatorTalavera-Núñez, María Elena-
Autor(es): dc.creatorDel-Aguila-Arcentales, Shyla-
Autor(es): dc.creatorYáñez, Jaime A.-
Autor(es): dc.creatorPonce-Soto, Luis Alberto-
Data de aceite: dc.date.accessioned2025-08-21T20:56:47Z-
Data de disponibilização: dc.date.available2025-08-21T20:56:47Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2022-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3389/fchem.2023.1217329-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/298417-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/298417-
Descrição: dc.descriptionObjective: The current study’s objective is to characterize a new throm-bin-like enzyme called TLBro that was obtained from Bothrops roedingeris snake from a biochemical and hemostatic perspective. Methodology: One chromatographic step was used to purify it, producing the serine protease TLBro. Molecular mass was estimated by SDS-PAGE to be between reduced and unreduced by 35 kDa. Tryptic peptide sequencing using Swiss Prot provided the complete amino acid sequence. Expasy.org by conducting a search that is limited to Crotalinae snake serine proteases and displaying a high degree of amino acid sequence. Results: Ser (182) is inhibited by phenylmethylsulfonyl fluoride (PMSF), and TLBro demonstrated the presence of Asp (88) residues. It also deduced the positions of His (43) and Ser (182) in the set of three coordinated amino acids in serine proteases. It was discovered that this substrate had high specificity for BANA, Michaelis-Menten behavior with KM 0 point85 mM and Vmax 1 point89 nmoles -NA/L/min, and high stability between temperatures (15 to 70°C) and pHs (2 point0 to 10 point0). According to doses and incubation times, TLBro degraded fibrin preferentially on the B-chain; additionally, its activities were significantly diminished after preincubation with divalent ions (Zn2 and Cd2). When incubated with PMSF, a particular serine protease inhibitor, enzymatic activities and platelet aggregation were inhibited. Conclusion: The findings revealed distinct structural and functional differences between the serine proteases, adding to the information and assisting in the improvement of the structure-function relationship.-
Descrição: dc.descriptionDepartment of Biochemistry Institute of Biology (IB) State University of Campinas (UNICAMP), SP-
Descrição: dc.descriptionFacultad de Administración y Negocios Universidad Tecnológica del Perú-
Descrição: dc.descriptionCentro de Estudos de Venenos e Animais Peçonhentos (CEVAP) Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), SP-
Descrição: dc.descriptionLaboratorio de Química de Proteínas Universidad Católica de Santa María-
Descrição: dc.descriptionDepartamento Académico de Química Facultad de Ciencias Naturales y Formales Universidad Nacional de San Agustín de Arequipa-
Descrição: dc.descriptionFacultad de Medicina Universidad Nacional de San Agustín de Arequipa-
Descrição: dc.descriptionEscuela de Posgrado Universidad San Ignacio de Loyola-
Descrição: dc.descriptionFacultad de Educación Carrera de Educación y Gestión del Aprendizaje Universidad Peruana de Ciencias Aplicadas-
Descrição: dc.descriptionCentro de Estudos de Venenos e Animais Peçonhentos (CEVAP) Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), SP-
Idioma: dc.languageen-
Relação: dc.relationFrontiers in Chemistry-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBothrops roedingeris-
Palavras-chave: dc.subjectprimary sequence-
Palavras-chave: dc.subjectserine protease-
Palavras-chave: dc.subjectsnake venom-
Palavras-chave: dc.subjectthrombin-like enzyme-
Palavras-chave: dc.subjectTLBro-
Título: dc.titleBiochemical and hemostatic description of a thrombin-like enzyme TLBro from Bothrops roedingeri snake venom-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.