In Situ Printing of Polylactic Acid/Nanoceramic Filaments for the Repair of Bone Defects Using a Portable 3D Device

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUFPI−Federal University of Piauí-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorScience and Technology (IFPI)-
Autor(es): dc.contributorState University of Piauí-
Autor(es): dc.creatorBrito, Guilherme Castro-
Autor(es): dc.creatorSousa, Gustavo Fernandes-
Autor(es): dc.creatorSantana, Moises Virgens-
Autor(es): dc.creatorAguiar Furtado, André Sales-
Autor(es): dc.creatorE Silva, Millena de Cassia Sousa-
Autor(es): dc.creatorLima Verde, Thiago Ferreira Candido-
Autor(es): dc.creatorBarbosa, Renata-
Autor(es): dc.creatorAlves, Tatianny Soares-
Autor(es): dc.creatorReis Vasconcellos, Luana Marotta-
Autor(es): dc.creatorSobral Silva, Leonardo Alvares-
Autor(es): dc.creatorFreitas Viana, Vicente Galber-
Autor(es): dc.creatorFigueredo-Silva, José-
Autor(es): dc.creatorMaia Filho, Antônio Luiz Martins-
Autor(es): dc.creatorMarciano, Fernanda Roberta-
Autor(es): dc.creatorLobo, Anderson Oliveira-
Data de aceite: dc.date.accessioned2025-08-21T20:58:16Z-
Data de disponibilização: dc.date.available2025-08-21T20:58:16Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2025-03-05-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1021/acsami.4c05232-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/298352-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/298352-
Descrição: dc.descriptionIn situ 3D printing is attractive for the direct repair of bone defects in underdeveloped countries and in emergency situations. So far, the lack of an interesting method to produce filament using FDA-approved biopolymers and nanoceramics combined with a portable strategy limits the use of in situ 3D printing. Herein, we investigated the osseointegration of new nanocomposite filaments based on polylactic acid (PLA), laponite (Lap), and hydroxyapatite (Hap) printed directly at the site of the bone defect in rats using a portable 3D printer. The filaments were produced using a single-screw extruder (L/D = 26), without the addition of solvents that can promote the toxicity of the materials. In vitro performance was evaluated in the cell differentiation process with mesenchymal stem cells (MSC) by an alkaline phosphatase activity test and visualization of mineralization nodules; a cell viability test and total protein dosage were performed to evaluate cytotoxicity. For the in vivo analysis, the PLA/Lap composite filaments with a diameter of 1.75 mm were printed directly into bone defects of Wistar rats using a commercially available portable 3D printer. Based on the in vitro and in vivo results, the in situ 3D printing technique followed by rapid cooling proved to be promising for bone tissue engineering. The absence of fibrous encapsulation and inflammatory processes became a good indicator of effectiveness in terms of biocompatibility parameters and bone tissue formation, and the use of the portable 3D printer showed a significant advantage in the application of this material by in situ printing.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionLIMAV−Interdisciplinary Laboratory for Advanced Materials BioMatLab Materials Science & Engineering Graduate Program UFPI−Federal University of Piauí, Piauí-
Descrição: dc.descriptionLAPCON─Laboratory of Polymers Conjugated Materials Technology Center CT Materials Science & Engineering Graduate Program UFPI−Federal University of Piauí, Piauí-
Descrição: dc.descriptionInstitute of Science and Technology São Paulo State University (UNESP), São Paulo 777 Eng. Francisco José Longo Avenue-
Descrição: dc.descriptionPostgraduate Program in Materials Engineering Federal Institute of Education Science and Technology (IFPI) Campus Teresina Central, Piauí-
Descrição: dc.descriptionBiotechnology Research Center State University of Piauí, Piauí-
Descrição: dc.descriptionDepartment of Physics UFPI−Federal University of Piauí, Piauí-
Descrição: dc.descriptionInstitute of Science and Technology São Paulo State University (UNESP), São Paulo 777 Eng. Francisco José Longo Avenue-
Formato: dc.format13135-13145-
Idioma: dc.languageen-
Relação: dc.relationACS Applied Materials and Interfaces-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subject3D printing-
Palavras-chave: dc.subjectbone repair-
Palavras-chave: dc.subjectemergency cases-
Palavras-chave: dc.subjectHydroxyapatite-
Palavras-chave: dc.subjectlaponite-
Título: dc.titleIn Situ Printing of Polylactic Acid/Nanoceramic Filaments for the Repair of Bone Defects Using a Portable 3D Device-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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