Phenol-free in-house kit for RNA extraction with applicability to SARS-CoV-2 genomic sequencing studies: A contribution to biotechnological sovereignty in Colombia

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidad de Córdoba-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversity of Cartagena-
Autor(es): dc.contributorUniversity of Sucre-
Autor(es): dc.contributorUniversidad CES-
Autor(es): dc.contributorWashington State University-
Autor(es): dc.contributorCorporación Universitaria del Caribe-CECAR-
Autor(es): dc.creatorGastelbondo-Pastrana, Bertha-
Autor(es): dc.creatorFlórez, Luis-
Autor(es): dc.creatorGuzmán, Camilo-
Autor(es): dc.creatorTorres, Karina-
Autor(es): dc.creatorGaray, Evelin-
Autor(es): dc.creatorBallesteros-Villamizar, Jolaime-
Autor(es): dc.creatorGutierrez, Rosa-
Autor(es): dc.creatorla Hoz, Daniel Echeverri-De-
Autor(es): dc.creatorLópez, Yésica-
Autor(es): dc.creatorContreras, Héctor-
Autor(es): dc.creatorArrieta, Germán-
Autor(es): dc.creatorSerrano-Coll, Héctor-
Autor(es): dc.creatorMartínez, Caty-
Autor(es): dc.creatorPájaro-Castro, Nérlis-
Autor(es): dc.creatorArroyo-Salgado, Bárbara-
Autor(es): dc.creatorRivero-Herrera, Ricardo-
Autor(es): dc.creatorHurtado, Eliana-
Autor(es): dc.creatorAraújo Jr., João Pessoa-
Autor(es): dc.creatorMattar, Salim-
Data de aceite: dc.date.accessioned2025-08-21T20:10:27Z-
Data de disponibilização: dc.date.available2025-08-21T20:10:27Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2025-05-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.jviromet.2025.115116-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/298300-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/298300-
Descrição: dc.descriptionDuring the COVID-19 pandemic, reagents for SARS-CoV-2 detection were scarce or sold at high prices, particularly in Latin America. In this study, a significant step towards self-sufficiency was achieved through the development of an in-house extraction kit for detecting SARS-CoV-2 from nasopharyngeal swab samples. The purity and concentration of the RNA extracted using the in-house kit were compared to those obtained using the GeneJET RNA Purification Kit (Thermo-Scientific®) as a reference. The applicability of the RNA extracted using the kit was evaluated using four samples positive for SARS-CoV-2 by NGS sequencing with Illumina®. There were no significant differences between the results obtained with the in-house kit and those obtained with the commercial kit. These findings confirm that the in-house protocol demonstrated satisfactory diagnostic accuracy for detecting the virus in patients with COVID-19. The in-house extraction kit works effectively, providing optimal RNA extraction for genomic characterization and lineage assignment of SARS-CoV-2 within the four positive samples analyzed. This phenol-free kit represents a local design and production achievement, offering an effective solution for RNA extraction and detection and sequencing of SARS-CoV-2 from nasopharyngeal swabs. The data highlight the essential contribution of this study to health and biotechnological sovereignty in Colombia.-
Descrição: dc.descriptionInstituto de Investigaciones Biológicas del Trópico Universidad de Córdoba Campus Berastegui-
Descrição: dc.descriptionSão Paulo State University (UNESP) Institute of Biotechnology (IBTEC), SP-
Descrição: dc.descriptionBiomedics Toxicology and Environmental Research Group-BIOTOXAM University of Cartagena-
Descrição: dc.descriptionDepartment of Medicine School of Health Sciences Medical and Pharmaceutical Sciences Group University of Sucre, Sucre-
Descrição: dc.descriptionInstituto Colombiano de Medicina Tropical Universidad CES-
Descrição: dc.descriptionPaul G. Allen School for Global Health Washington State University-
Descrição: dc.descriptionGrupo de Investigaciones Microbiológicas y Biomédicas de Córdoba-GIMBIC Universidad de Córdoba-
Descrição: dc.descriptionGrupo de Salud Pública y Auditoría en Salud Corporación Universitaria del Caribe-CECAR-
Descrição: dc.descriptionGrupo de Investigación Evaluación y Desarrollo de Fármacos y Afines - IDEFARMA Universidad de Córdoba-
Descrição: dc.descriptionSão Paulo State University (UNESP) Institute of Biotechnology (IBTEC), SP-
Idioma: dc.languageen-
Relação: dc.relationJournal of Virological Methods-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCOVID-19 testing-
Palavras-chave: dc.subjectDeveloping countries-
Palavras-chave: dc.subjectEconomic development-
Palavras-chave: dc.subjectInnovation-
Palavras-chave: dc.subjectMolecular Diagnostic Techniques-
Palavras-chave: dc.subjectResearch and Development-
Título: dc.titlePhenol-free in-house kit for RNA extraction with applicability to SARS-CoV-2 genomic sequencing studies: A contribution to biotechnological sovereignty in Colombia-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.