Toxicological Assessment of 2-Hydroxychalcone-Mediated Photodynamic Therapy: Comparative In Vitro and In Vivo Approaches

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Estadual de Maringá (UEM)-
Autor(es): dc.creatorBila, Níura Madalena-
Autor(es): dc.creatorVaso, Carolina Orlando-
Autor(es): dc.creatorBelizário, Jenyffie Araújo-
Autor(es): dc.creatorAssis, Letícia Ribeiro-
Autor(es): dc.creatorRegasini, Luís Octávio-
Autor(es): dc.creatorFontana, Carla Raquel-
Autor(es): dc.creatorFusco-Almeida, Ana Marisa-
Autor(es): dc.creatorCosta-Orlandi, Caroline Barcelos-
Autor(es): dc.creatorMendes-Giannini, Maria José Soares-
Data de aceite: dc.date.accessioned2025-08-21T16:14:57Z-
Data de disponibilização: dc.date.available2025-08-21T16:14:57Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/pharmaceutics16121523-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/298297-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/298297-
Descrição: dc.descriptionBackground: Photodynamic therapy (PDT) is a treatment modality that uses light to activate a photosensitizing agent, destroying target cells. The growing awareness of the necessity to reduce or eliminate the use of mammals in research has prompted the search for safer toxicity testing models aligned with the new global guidelines and compliant with the relevant regulations. Objective: The objective of this study was to assess the impact of PDT on alternative models to mammals, including in vitro three-dimensional (3D) cultures and in vivo, in invertebrate animals, utilizing a potent photosensitizer, 2-hydroxychalcone. Methods: Cytotoxicity was assessed in two cellular models: monolayer (2D) and 3D. For this purpose, spheroids of two cell lines, primary dermal fibroblasts (HDFa) and adult human epidermal cell keratinocytes (HaCat), were developed and characterized following criteria on cell viability, shape, diameter, and number of cells. The survival percentages of Caenorhabditis elegans and Galleria mellonella were evaluated at 1 and 7 days, respectively. Results: The findings indicated that all the assessed platforms are appropriate for investigating PDT toxicity. Furthermore, 2-hydroxychalcone demonstrated low toxicity in the absence of light and when mediated by PDT across a range of in vitro (2D and 3D cultures) and in vivo (invertebrate animal models, including G. mellonella and C. elegans) models. Conclusion: There was a strong correlation between the in vitro and in vivo tests, with similar toxicity results, particularly in the 3D models and C. elegans, where the concentration for 50% viability was approximately 100 µg/mL.-
Descrição: dc.descriptionDepartment of Clinical Analysis School of Pharmaceutical Sciences Universidade Estadual Paulista (UNESP), SP-
Descrição: dc.descriptionDepartment of Public Health School of Veterinary Universidade Eduardo Modlane (UEM), Maputo 257-
Descrição: dc.descriptionDepartment of Chemistry and Environmental Sciences Institute of Biosciences Humanities and Exact Sciences Universidade Estaudal Paulista (UNESP), SP-
Descrição: dc.descriptionDepartment of Clinical Analysis School of Pharmaceutical Sciences Universidade Estadual Paulista (UNESP), SP-
Descrição: dc.descriptionDepartment of Chemistry and Environmental Sciences Institute of Biosciences Humanities and Exact Sciences Universidade Estaudal Paulista (UNESP), SP-
Idioma: dc.languageen-
Relação: dc.relationPharmaceutics-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subject2-hydroxychalcone-
Palavras-chave: dc.subjectCaenorhabditis elegans-
Palavras-chave: dc.subjectGalleria mellonella-
Palavras-chave: dc.subjectmonolayers-
Palavras-chave: dc.subjectphotodynamic therapy-
Palavras-chave: dc.subjectthree-dimensional tissue model-
Palavras-chave: dc.subjecttoxicological evaluation of compounds-
Título: dc.titleToxicological Assessment of 2-Hydroxychalcone-Mediated Photodynamic Therapy: Comparative In Vitro and In Vivo Approaches-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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