Modeling reproductive and pregnancy-associated tissues using organ-on-chip platforms: challenges, limitations, and the high throughput data frontier

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Autor(es): dc.contributorUniversity Blvd.-
Autor(es): dc.contributorUniversity of the Philippines Manila-
Autor(es): dc.contributorThe University of Texas Medical Branch at Galveston-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorSan Beda University-
Autor(es): dc.contributorDe La Salle University Manila-
Autor(es): dc.contributorTexas A&M University-
Autor(es): dc.contributorUniformed Services University of the Health Sciences-
Autor(es): dc.contributorInova Health System-
Autor(es): dc.creatorTruong, Nina-
Autor(es): dc.creatorZahra, Abir-
Autor(es): dc.creatorLintao, Ryan C. V.-
Autor(es): dc.creatorChauhan, Rahul-
Autor(es): dc.creatorBento, Giovana Fernanda-
Autor(es): dc.creatorVidal Jr, Manuel-
Autor(es): dc.creatorKim, Sungjin-
Autor(es): dc.creatorLam, Po Yi-
Autor(es): dc.creatorConrads, Thomas-
Autor(es): dc.creatorConrads, Kelly-
Autor(es): dc.creatorHan, Arum-
Autor(es): dc.creatorMenon, Ramkumar-
Autor(es): dc.creatorRichardson, Lauren S.-
Data de aceite: dc.date.accessioned2025-08-21T17:14:30Z-
Data de disponibilização: dc.date.available2025-08-21T17:14:30Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3389/fbioe.2025.1568389-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/298295-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/298295-
Descrição: dc.descriptionOver the past decade, organ-on-chip technology (microphysiological systems or tissue chips) has reshaped in-vitro physiological and pathological modeling and pharmaceutical drug assessment. FDA Modernization Act 2.0 allows for alternatives to animal testing or the use of appropriate non-animal models/new approach methods (NAMs), such as Organ-on-chips (OC) platforms or in silico simulation models, to generate pre-clinical in-vitro drug trial data for regulatory purposes primes the microfluidic field to have exponential growth in the coming years. The changes in the approaches of regulatory agencies could significantly impact the development of therapeutics for use during pregnancy. However, limitations of the devices and molecular and biochemical assay shortfalls hinder the progress of the OOC field. This review describes available reproductive and pregnancy-related OOC platforms, and the current methodologies utilized to generate endpoint datasets (e.g., microscopic imaging, immunocytochemistry, real-time polymerase chain reaction, cytokine multiplex analysis). Microfluidic platform limitations, such as fewer number of cells or low supernatant volumes and restrictions regarding fabrication materials, are described. Novel approaches (e.g., spatial transcriptomics, imaging cytometry by time of flight, exosomes analysis using Exoview) to overcome these challenges are described. OOC platforms are primed to provide biologically relevant and clinically translational data that can revolutionize in-vitro physiological modeling, drug discovery, and toxicologic risk assessment. However, engineering adaptations to increase the throughput of devices (i.e., device arrays) and biological advancements to improve data throughput are both needed for these platforms to reach their full potential.-
Descrição: dc.descriptionNational Institute of Child Health and Human Development-
Descrição: dc.descriptionJohn Sealy School of Medicine University Blvd.-
Descrição: dc.descriptionInstitute of Reproductive Health National Institutes of Health University of the Philippines Manila-
Descrição: dc.descriptionDivision of Basic Science and Translational Research Department of Obstetrics and Gynecology The University of Texas Medical Branch at Galveston-
Descrição: dc.descriptionDepartment of Pathology Botucatu Medical School São Paulo State University-
Descrição: dc.descriptionCollege of Medicine San Beda University-
Descrição: dc.descriptionDepartment of Chemistry College of Science De La Salle University Manila-
Descrição: dc.descriptionDepartment of Biomedical Engineering and Electrical and Computer Engineering Texas A&M University, College Station-
Descrição: dc.descriptionGynecologic Cancer Center of Excellence Gynecologic Surgery and Obstetrics Uniformed Services University of the Health Sciences-
Descrição: dc.descriptionWomen’s Health Integrated Research Center Women’s Service Line Inova Health System-
Descrição: dc.descriptionDepartment of Pathology Botucatu Medical School São Paulo State University-
Descrição: dc.descriptionNational Institute of Child Health and Human Development: UG3TR003283 R01HD110400 R01HD100729-01S1-
Idioma: dc.languageen-
Relação: dc.relationFrontiers in Bioengineering and Biotechnology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectcell culture-
Palavras-chave: dc.subjectin-vitro tools-
Palavras-chave: dc.subjectorgan-on-chip-
Palavras-chave: dc.subjectpregnancy-
Palavras-chave: dc.subjectreproduction-
Título: dc.titleModeling reproductive and pregnancy-associated tissues using organ-on-chip platforms: challenges, limitations, and the high throughput data frontier-
Tipo de arquivo: dc.typevídeo-
Aparece nas coleções:Repositório Institucional - Unesp

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