Oocyte Competence of Prepubertal Sheep and Goat Oocytes: An Assessment of Large-Scale Chromatin Configuration and Epidermal Growth Factor Receptor Expression in Oocytes and Cumulus Cells

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Autor(es): dc.contributorAutonomous University of Barcelona-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorFerrer-Roda, Mònica-
Autor(es): dc.creatorIzquierdo, Dolors-
Autor(es): dc.creatorGil, Ana-
Autor(es): dc.creatorOliveira, Maria Emilia Franco-
Autor(es): dc.creatorParamio, Maria-Teresa-
Data de aceite: dc.date.accessioned2025-08-21T15:33:26Z-
Data de disponibilização: dc.date.available2025-08-21T15:33:26Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-04-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/ijms25084474-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/298294-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/298294-
Descrição: dc.descriptionThe oocyte competence of prepubertal females is lower compared to that of adults, mainly because they originate from small follicles. In adult females, the germinal vesicle (GV) and epidermal growth factor receptor (EGFR) have been associated with oocyte competence. This study aimed to analyze GV chromatin configuration and EGFR expression in prepubertal goat and sheep oocytes obtained from small (<3 mm) and large (≥3 mm) follicles and compare them with those from adults. GV chromatin was classified from diffuse to condensed as GV1, GVn, and GVc for goats and NSN, SN, and SNE for sheep. EGFR was quantified in cumulus cells (CCs) by Western blotting and in oocytes by immunofluorescence. Oocytes from prepubertal large follicles and adults exhibited highly condensed chromatin in goats (71% and 69% in GVc, respectively) and sheep (59% and 75% in SNE, respectively). In both species, EGFR expression in CCs and oocytes was higher in prepubertal large follicles than in small ones. In adult females, EGFR expression in oocytes was higher than in prepubertal large follicles. In conclusion, GV configuration and EGFR expression in CCs and oocytes were higher in the large than small follicles of prepubertal females.-
Descrição: dc.descriptionGeneralitat de Catalunya-
Descrição: dc.descriptionDepartment of Animal and Food Science Veterinary Faculty Autonomous University of Barcelona-
Descrição: dc.descriptionSchool of Agricultural and Veterinarian Sciences São Paulo State University-
Descrição: dc.descriptionSchool of Agricultural and Veterinarian Sciences São Paulo State University-
Descrição: dc.descriptionGeneralitat de Catalunya: 2020 FI-B 00025-
Idioma: dc.languageen-
Relação: dc.relationInternational Journal of Molecular Sciences-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectEGF receptor-
Palavras-chave: dc.subjectgerminal vesicle-
Palavras-chave: dc.subjectgoat-
Palavras-chave: dc.subjectoocyte-
Palavras-chave: dc.subjectsheep-
Título: dc.titleOocyte Competence of Prepubertal Sheep and Goat Oocytes: An Assessment of Large-Scale Chromatin Configuration and Epidermal Growth Factor Receptor Expression in Oocytes and Cumulus Cells-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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