Osteoprotective effect by interleukin-4 (IL-4) on lipoprotein-induced periodontitis

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorSahlgrenska Academy at University of Gothenburg-
Autor(es): dc.contributorFederal University of Maranhão – UFMA-
Autor(es): dc.contributorUniversidade Federal de Uberlândia (UFU)-
Autor(es): dc.contributorUniversidade Federal de Goiás (UFG)-
Autor(es): dc.creatorLima Teixeira, Jorge F.-
Autor(es): dc.creatorHenning, Petra-
Autor(es): dc.creatorCintra Magalhães, Fernando A.-
Autor(es): dc.creatorColetto-Nunes, Glaucia-
Autor(es): dc.creatorFloriano-Marcelino, Thais-
Autor(es): dc.creatorWesterlund, Anna-
Autor(es): dc.creatorMovérare-Skrtic, Sofia-
Autor(es): dc.creatorOliveira, Guilherme J.P.L.-
Autor(es): dc.creatorLerner, Ulf H.-
Autor(es): dc.creatorSouza, Pedro Paulo C.-
Data de aceite: dc.date.accessioned2025-08-21T22:30:08Z-
Data de disponibilização: dc.date.available2025-08-21T22:30:08Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.cyto.2023.156399-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/298289-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/298289-
Descrição: dc.descriptionLipoproteins are immunostimulatory bacterial components suggested to participate in inflammation-induced bone loss in periodontal disease through stimulation of osteoclast differentiation. Toll-like receptor 2 activation by Pam2CSK4 (PAM2), known to mimic bacterial lipoproteins, was previously shown to enhance periodontal bone resorption in mice. The anti-inflammatory cytokine interleukin-4 (IL-4) is a known inhibitor of RANKL-induced bone resorption in vitro. Here, we have investigated whether IL-4 could decrease PAM2-induced periodontal bone loss and osteoclastogenesis in vivo. In a model of periodontitis induced by gingival injections of PAM2 in mice, concomitant injections of IL-4 reduced bone loss. Histologically, IL-4 reduced the recruitment of inflammatory cells and the formation of TRAP+ osteoclasts stimulated by PAM2. Mouse bone marrow macrophages (BMMs) and neonatal calvarial osteoblasts were used to assess the effect of IL-4 on PAM2-induced osteoclastogenesis in vitro. In RANKL-primed BMMs stimulated by PAM2 Nfatc1, Ctsk, and Acp5 gene expression was up-regulated and resulted in robust formation of TRAP+ multinucleated osteoclasts, effects which were impaired by IL-4. These effects were mediated by impairment in PAM2-induced c-fos expression. In primary calvarial osteoblast cultures, IL-4 decreased PAM2-induced Tnfsf11 (encoding RANKL) mRNA and enhanced Tnfrsf11b (encoding OPG) expression. Our data demonstrate that the osteoprotective effect by IL-4 on lipoprotein-induced periodontal disease occurs through the inhibition of osteoclastogenesis by three mechanisms, one by acting directly on osteoclast progenitors, another by acting indirectly through decreasing the expression of osteoclast-regulating cytokines in osteoblasts and a third by decreasing inflammation.-
Descrição: dc.descriptionDepartment of Pathology and Physiology School of Dentistry at Araraquara Univ. Est. Paulista – UNESP-
Descrição: dc.descriptionSahlgrenska Osteoporosis Centre and Centre for Bone and Arthritis Research at Department of Internal Medicine and Clinical Nutrition Institute for Medicine Sahlgrenska Academy at University of Gothenburg-
Descrição: dc.descriptionDepartment of Pathology School of Nursing Federal University of Maranhão – UFMA-
Descrição: dc.descriptionDepartment of Periodontology and Implantodontology Dental School Federal University of Uberlândia – UFU-
Descrição: dc.descriptionInnovation in Biomaterials Laboratory (iBioM) Faculty of Dentistry Federal University of Goiás – UFG-
Descrição: dc.descriptionDepartment of Pathology and Physiology School of Dentistry at Araraquara Univ. Est. Paulista – UNESP-
Idioma: dc.languageen-
Relação: dc.relationCytokine-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBone resorption-
Palavras-chave: dc.subjectIL-4-
Palavras-chave: dc.subjectInflammation-
Palavras-chave: dc.subjectOsteoclasts-
Palavras-chave: dc.subjectPeriodontitis-
Título: dc.titleOsteoprotective effect by interleukin-4 (IL-4) on lipoprotein-induced periodontitis-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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