Molecular diagnosis and characterization of Anaplasma marginale and Ehrlichia ruminantium infecting beef cattle of Maputo Province, Mozambique

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorDirecção de Ciências Animais-
Autor(es): dc.contributorUniversidade Pedagógica Maputo-
Autor(es): dc.contributorImunodot Diagnósticos Veterinários – IMUNODOT-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorMatos, Carlos António-
Autor(es): dc.creatorNomboro, Cesária Fiossiane-
Autor(es): dc.creatorGonçalves, Luiz Ricardo-
Autor(es): dc.creatorCala, Aida Cristina-
Autor(es): dc.creatorSitoe, Carlos Francisco-
Autor(es): dc.creatorVinte, Ana Paula Rúpia-
Autor(es): dc.creatorMondlane, Cristovão Mario-
Autor(es): dc.creatorAndré, Marcos Rogério-
Autor(es): dc.creatordo Carmo Carrilho, Maria-
Data de aceite: dc.date.accessioned2025-08-21T20:24:33Z-
Data de disponibilização: dc.date.available2025-08-21T20:24:33Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1186/s12917-024-04045-4-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/298268-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/298268-
Descrição: dc.descriptionBackground: Members of the Anaplasmataceae family, such as the Anaplasma and Ehrlichia species, cause economic losses and public health risks. However, the exact economic impact has not been comprehensively assessed in Mozambique due to limited data available on its basic epidemiology. Therefore, we investigated the molecular occurrence and identity of Anaplasma and Ehrlichia spp. infecting beef cattle in Maputo province, Mozambique. Methods: A total of 200 whole blood samples were collected from apparently healthy beef cattle. Whole blood DNA was extracted and tested for presence of Anaplasma spp. and Ehrlichia ruminantium DNA through amplification of the 16S rRNA and map1 genes. Positive samples to Anaplasma spp. were subject to PCR assay targeting the A. marginale-msp5 gene. Amplicons obtained were purified, sequenced and subject to phylogenetic analyses. Results: Anaplasma spp., A. marginale and E. ruminantium were detected in 153 (76.5%), 142 (71%) and 19 (9.5%) of all the samples analyzed, respectively. On this same sample group, 19 (9.5%) were co-infected with A. marginale and E. ruminantium. The 16S rRNA sequences of Anaplasma spp. obtained were phylogenetically related to A. marginale, A. centrale and A. platys. Phylogenetic analysis revealed that A. marginale-msp5 nucleotide sequences were grouped with sequences from Asia, Africa and Latin America, whereas E. ruminantium-map1 DNA nucleotide sequences were positioned in multiple clusters. Conclusion: Cattle in Maputo Province are reservoirs for multiple Anaplasma species. A high positivity rate of infection by A. marginale was observed, as well as high genetic diversity of E. ruminantium. Furthermore, five new genotypes of E. ruminantium-map1 were identified.-
Descrição: dc.descriptionLaboratório de Parasitologia Direcção de Ciências Animais, Avenida de Moçambique, km 1.5, Bairro do Jardim-
Descrição: dc.descriptionUniversidade Pedagógica Maputo-
Descrição: dc.descriptionImunodot Diagnósticos Veterinários – IMUNODOT, SP-
Descrição: dc.descriptionVector-Borne Bioagents Laboratory (VBBL) Departamento de Patologia Reprodução e Saúde Única São Paulo State University (UNESP) School of Agricultural and Veterinary Sciences Jaboticabal, SP-
Descrição: dc.descriptionVector-Borne Bioagents Laboratory (VBBL) Departamento de Patologia Reprodução e Saúde Única São Paulo State University (UNESP) School of Agricultural and Veterinary Sciences Jaboticabal, SP-
Idioma: dc.languageen-
Relação: dc.relationBMC Veterinary Research-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subject16S rRNA-
Palavras-chave: dc.subjectAnaplasmaspp-
Palavras-chave: dc.subjectCattle-
Palavras-chave: dc.subjectEhrlichia ruminantium-
Palavras-chave: dc.subjectMap1-
Palavras-chave: dc.subjectMaputo Province-
Palavras-chave: dc.subjectmsp5-
Título: dc.titleMolecular diagnosis and characterization of Anaplasma marginale and Ehrlichia ruminantium infecting beef cattle of Maputo Province, Mozambique-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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