In Vitro Activity of Quaternary Ammonium in Prototheca Isolated from Clinical Bovine Mastitis Identified by Mass Spectrometry and PCR Sequencing of the cytb Gene Marker

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversity of Warsaw-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorArabe Filho, Marcelo Fagali-
Autor(es): dc.creatorJagielski, Tomasz-
Autor(es): dc.creatorProskurnicka, Angelika-
Autor(es): dc.creatorSantos, Marcos Veiga dos-
Autor(es): dc.creatorFidelis, Carlos Eduardo-
Autor(es): dc.creatorGuimarães, Felipe Freitas-
Autor(es): dc.creatorGuerra, Simony Trevizan-
Autor(es): dc.creatorJoaquim, Sâmea Fernandes-
Autor(es): dc.creatorMioni, Mateus de Souza Ribeiro-
Autor(es): dc.creatorPantoja, José Carlos de Figueiredo-
Autor(es): dc.creatorLangoni, Helio-
Autor(es): dc.creatorSanchez, Luísa Fernanda García-
Autor(es): dc.creatorRibeiro, Marcio Garcia-
Data de aceite: dc.date.accessioned2025-08-21T19:00:27Z-
Data de disponibilização: dc.date.available2025-08-21T19:00:27Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-10-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/ani13203286-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/298196-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/298196-
Descrição: dc.descriptionThe in vitro algaecide activity of quaternary ammonium (QA) against Prototheca isolated from bovine clinical mastitis was investigated, in which the clinical severity was scored, milk samples were subjected to microbiological culture, and algal species were identified by molecular typing. A total of 4275 milk clinical samples of different cows from ten large dairy farms were used. Forty-four (1%) samples of cows from three dairy farms yielded growth of Prototheca, of which 88.6% (39/44) were identified as Prototheca bovis and 11.3% (5/44) as Prototheca sp. by MALDI-TOF MS, whereas 100% of the isolates were identified as P. bovis using PCR sequencing of the cytb gene. Among cows for which clinical severity scoring was available, 78.8% (26/33) and 21.2% (7/33) had mild and moderate infections, respectively, whereas no animal showed severe clinical signs. The algaecide activity of QA in Prototheca was observed in low concentrations among all isolates, in 20.4% (9/44) at 35 ppm, 36.4% (16/44) at 17 ppm, and 43.2% (19/44) at an 8 ppm, in addition to activity on three reference Prototheca strains. Overall, the study highlights the predominance of P. bovis as the causative agent of algal mastitis in bovines. Prototheca induced abnormalities preponderantly in the milk and mammary gland tissue of cows, and to our knowledge, our study is the first to apply clinical severity scoring in protothecal mastitis. In addition, the study underlines the activity of QA in low concentrations against Prototheca, indicating its potential use as an antiseptic/disinfectant in milking facilities and dairy environments.-
Descrição: dc.descriptionDepartment of Animal Production and Preventive Veterinary Medicine School of Veterinary Medicine and Animal Science Sao Paulo State University—UNESP, SP-
Descrição: dc.descriptionDepartment of Medical Microbiology Institute of Microbiology Faculty of Biology University of Warsaw-
Descrição: dc.descriptionDepartment of Animal Nutrition and Production School of Veterinary Medicine and Animal Science University of Sao Paulo—USP, SP-
Descrição: dc.descriptionDepartment of Pathology Reproduction and One Health Sao Paulo State University—UNESP, SP-
Descrição: dc.descriptionDepartment of Animal Production and Preventive Veterinary Medicine School of Veterinary Medicine and Animal Science Sao Paulo State University—UNESP, SP-
Descrição: dc.descriptionDepartment of Pathology Reproduction and One Health Sao Paulo State University—UNESP, SP-
Idioma: dc.languageen-
Relação: dc.relationAnimals-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectalgaecide compounds-
Palavras-chave: dc.subjectclinical severity scoring-
Palavras-chave: dc.subjectintramammary protothecosis-
Palavras-chave: dc.subjectmolecular typing-
Palavras-chave: dc.subjectPrototheca bovis-
Título: dc.titleIn Vitro Activity of Quaternary Ammonium in Prototheca Isolated from Clinical Bovine Mastitis Identified by Mass Spectrometry and PCR Sequencing of the cytb Gene Marker-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.