Mesoscale characterization of osseointegration around an additively manufactured genistein-coated implant

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Autor(es): dc.contributorMcMaster University-
Autor(es): dc.contributorUniversity of Gothenburg-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorResearch Productivity Scholarship (Process: 309408/2020-2)-
Autor(es): dc.creatorMicheletti, Chiara-
Autor(es): dc.creatorDiCecco, Liza-Anastasia-
Autor(es): dc.creatorDeering, Joseph-
Autor(es): dc.creatorChen, Wanqi-
Autor(es): dc.creatorErvolino da Silva, Ana Cláudia-
Autor(es): dc.creatorShah, Furqan A.-
Autor(es): dc.creatorPalmquist, Anders-
Autor(es): dc.creatorOkamoto, Roberta-
Autor(es): dc.creatorGrandfield, Kathryn-
Data de aceite: dc.date.accessioned2025-08-21T20:28:53Z-
Data de disponibilização: dc.date.available2025-08-21T20:28:53Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1038/s41598-024-66058-1-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/298123-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/298123-
Descrição: dc.descriptionGiven the hierarchical nature of bone and bone interfaces, osseointegration, namely the formation of a direct bone-implant contact, is best evaluated using a multiscale approach. However, a trade-off exists between field of view and spatial resolution, making it challenging to image large volumes with high resolution. In this study, we combine established electron microscopy techniques to probe bone-implant interfaces at the microscale and nanoscale with plasma focused ion beam-scanning electron microscopy (PFIB-SEM) tomography to evaluate osseointegration at the mesoscale. This characterization workflow is demonstrated for bone response to an additively manufactured Ti-6Al-4V implant which combines engineered porosity to facilitate bone ingrowth and surface functionalization via genistein, a phytoestrogen, to counteract bone loss in osteoporosis. SEM demonstrated new bone formation at the implant site, including in the internal implant pores. At the nanoscale, scanning transmission electron microscopy and energy-dispersive X-ray spectroscopy confirmed the gradual nature of the bone-implant interface. By leveraging mesoscale analysis with PFIB-SEM tomography that captures large volumes of bone-implant interface with nearly nanoscale resolution, the presence of mineral ellipsoids varying in size and orientation was revealed. In addition, a well-developed lacuno-canalicular network and mineralization fronts directed both towards the implant and away from it were highlighted.-
Descrição: dc.descriptionAcademy of Osseointegration-
Descrição: dc.descriptionCentre for Antibiotic Resistance Research, University of Gothenburg-
Descrição: dc.descriptionSvenska Sällskapet för Medicinsk Forskning-
Descrição: dc.descriptionWallenberg Centre for Molecular and Translational Medicine-
Descrição: dc.descriptionGöteborgs Universitet-
Descrição: dc.descriptionStiftelsen Blanceflor Boncompagni Ludovisi, född Bildt-
Descrição: dc.descriptionVetenskapsrådet-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionCanadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of Canada-
Descrição: dc.descriptionNatural Sciences and Engineering Research Council of Canada-
Descrição: dc.descriptionDepartment of Materials Science and Engineering McMaster University-
Descrição: dc.descriptionDepartment of Biomaterials Sahlgrenska Academy University of Gothenburg-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery Araçatuba Dental School São Paulo State University, SP-
Descrição: dc.descriptionDepartment of Basic Sciences Araçatuba Dental School São Paulo State University, SP-
Descrição: dc.descriptionSchool of Biomedical Engineering McMaster University-
Descrição: dc.descriptionBrockhouse Institute for Materials Research McMaster University-
Descrição: dc.descriptionResearch Productivity Scholarship (Process: 309408/2020-2), SP-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery Araçatuba Dental School São Paulo State University, SP-
Descrição: dc.descriptionDepartment of Basic Sciences Araçatuba Dental School São Paulo State University, SP-
Descrição: dc.descriptionVetenskapsrådet: 2020-04715-
Descrição: dc.descriptionFAPESP: 2021/06849-4-
Descrição: dc.descriptionFAPESP: 2021/13026-4-
Descrição: dc.descriptionCanadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of Canada: ALLRP 576146-22-
Descrição: dc.descriptionNatural Sciences and Engineering Research Council of Canada: ALLRP 576146-22-
Idioma: dc.languageen-
Relação: dc.relationScientific Reports-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectLCN-
Palavras-chave: dc.subjectMineral ellipsoid-
Palavras-chave: dc.subjectOsseointegration-
Palavras-chave: dc.subjectPFIB-SEM tomography-
Palavras-chave: dc.subjectResin cast etching-
Palavras-chave: dc.subjectScanning transmission electron microscopy-
Título: dc.titleMesoscale characterization of osseointegration around an additively manufactured genistein-coated implant-
Tipo de arquivo: dc.typelivro digital-
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