A soybean trypsin inhibitor reduces the resistance to transgenic maize in a population of Spodoptera frugiperda (Lepidoptera: Noctuidae)

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorCorteva Agriscience-
Autor(es): dc.creatorFonseca, Sandy Sousa-
Autor(es): dc.creatorSantos, Ana Leticia Zero-
Autor(es): dc.creatorPinto, Ciro Pedro Guidotti-
Autor(es): dc.creatorMarques, Luiz-
Autor(es): dc.creatorSantos, Antonio Cesar-
Autor(es): dc.creatorBing, James-
Autor(es): dc.creatorNowatzki, Timothy-
Autor(es): dc.creatorSethi, Amit-
Autor(es): dc.creatorRossi, Guilherme Duarte-
Data de aceite: dc.date.accessioned2025-08-21T15:15:18Z-
Data de disponibilização: dc.date.available2025-08-21T15:15:18Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1093/jee/toad188-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/298087-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/298087-
Descrição: dc.descriptionLepidopteran pests have been successfully managed by the adoption of insect resistant transgenic plants expressing Cry and/or Vip insecticidal proteins derived from Bacillus thuringiensis (Bt plants). Among such pests, Spodoptera frugiperda (Smith, 1797) (Lepidoptera: Noctuidae) is highlighted for its destructive potential in maize crops and for cases of field-evolved resistance to Bt plants. Cry insecticidal proteins expressed in Bt plants are known for their interaction with insect midgut receptors and subsequent midgut cell disruption that leads to target pest death. In the midgut of lepidopteran larval pests such as S. frugiperda, serine proteases are important in dietary protein digestion and activation or degradation of insecticidal proteins. This work was conducted to evaluate if the use of a soybean trypsin inhibitor (SBTI) could disrupt the development of a Bt-susceptible and a Bt-resistant population of S. frugiperda ingesting Bt (expressing Cry1F, Cry1A.105, and Cry2Ab2 Cry proteins) and non-Bt maize plants. The SBTI was produced and purified using recombinant expression in E. coli followed by purification in Ni-Sepharose. Bioassays using non-Bt maize leaves indicated that the development of susceptible and resistant populations of S. frugiperda was not influenced by the ingestion of SBTI. However, when the resistant population consumed Bt maize plants amended with SBTI, high mortality along with a reduction in larval weight and reduced activity of digestive trypsins were observed. Although the mode of action was not elucidated, it is possible that the consumption of SBTI increased susceptibility to Bt maize in the resistant population of S. frugiperda.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionSão Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences, SP-
Descrição: dc.descriptionCorteva Agriscience, Avenida Tamboré, 267, Alphaville Barueri, SP-
Descrição: dc.descriptionCorteva Agriscience, 7000 NW 62nd Avenue-
Descrição: dc.descriptionSão Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences, SP-
Descrição: dc.descriptionCAPES: 001-
Formato: dc.format2146-2153-
Idioma: dc.languageen-
Relação: dc.relationJournal of Economic Entomology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectinsect nutrition-
Palavras-chave: dc.subjectinsect resistance management-
Palavras-chave: dc.subjecttrypsin inhibition-
Título: dc.titleA soybean trypsin inhibitor reduces the resistance to transgenic maize in a population of Spodoptera frugiperda (Lepidoptera: Noctuidae)-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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