Beyond Angiogenesis: The Multitasking Approach of the First PEGylated Vascular Endothelial Growth Factor (CdtVEGF) from Brazilian Rattlesnake Venom

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Federal da Bahia (UFBA)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorFerreira, Isabela-
Autor(es): dc.creatorOliveira, Isadora-
Autor(es): dc.creatorBordon, Karla-
Autor(es): dc.creatorReis, Mouzarllem-
Autor(es): dc.creatorWiezel, Gisele-
Autor(es): dc.creatorSanchez, Caroline-
Autor(es): dc.creatorSantos, Luísa-
Autor(es): dc.creatorSantos-Filho, Norival-
Autor(es): dc.creatorPucca, Manuela-
Autor(es): dc.creatorAntunes, Lusânia-
Autor(es): dc.creatorLopes, Daiana-
Autor(es): dc.creatorArantes, Eliane-
Data de aceite: dc.date.accessioned2025-08-21T18:26:36Z-
Data de disponibilização: dc.date.available2025-08-21T18:26:36Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-08-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/toxins15080483-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/298000-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/298000-
Descrição: dc.descriptionA pioneering study regarding the isolation, biochemical evaluation, functional assays and first PEGylation report of a novel vascular endothelial growth factor from Crotalus durissus terrificus venom (CdtVEGF and PEG-CdtVEGF). CdtVEGF was isolated from crude venom using two different chromatographic steps, representing 2% of soluble venom proteins. Its primary sequence was determined using mass spectrometry analysis, and the molecule demonstrated no affinity to heparin. The Brazilian crotalid antivenom recognized CdtVEGF. Both native and PEGylated CdtVEGF were able to induce new vessel formation and migration, and to increase the metabolic activity of human umbilical endothelial vascular cells (HUVEC), resulting in better wound closure (~50% within 12 h) using the native form. CdtVEGF induced leukocyte recruitment to the peritoneal cavity in mice, with a predominance of neutrophil influx followed by lymphocytes, demonstrating the ability to activate the immune system. The molecule also induced a dose-dependent increase in vascular permeability, and PEG-CdtVEGF showed less in vivo inflammatory activity than CdtVEGF. By unraveling the intricate properties of minor components of snake venom like svVEGF, this study illuminates the indispensable significance of exploring these molecular tools to unveil physiological and pathological processes, elucidates the mechanisms of snakebite envenomings, and could possibly be used to design a therapeutic drug.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionDepartment of BioMolecular Sciences School of Pharmaceutical Sciences of Ribeirão Preto University of Sao Paulo, SP-
Descrição: dc.descriptionDepartment of Clinical Analysis Toxicology and Food Science School of Pharmaceutical Sciences of Ribeirao Preto University of Sao Paulo, SP-
Descrição: dc.descriptionInstitute Multidisciplinary in Health Federal University of Bahia, BA-
Descrição: dc.descriptionDepartment of Biochemistry and Organic Chemistry Chemistry Institute Sao Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Clinical Analysis Sao Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Biochemistry and Organic Chemistry Chemistry Institute Sao Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Clinical Analysis Sao Paulo State University (UNESP), SP-
Descrição: dc.descriptionCNPq: 307155/2017-0-
Descrição: dc.descriptionCNPq: 309399/2021-1-
Idioma: dc.languageen-
Relação: dc.relationToxins-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCrotalus durissus terrificus-
Palavras-chave: dc.subjectneutrophilic recruitment-
Palavras-chave: dc.subjectPEGylation-
Palavras-chave: dc.subjectvascular permeability-
Palavras-chave: dc.subjectVEGF-
Título: dc.titleBeyond Angiogenesis: The Multitasking Approach of the First PEGylated Vascular Endothelial Growth Factor (CdtVEGF) from Brazilian Rattlesnake Venom-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.