Possible immunomodulatory role of Filifactor alocis through beta-defensin 2 in gingival keratinocytes

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversity Medical Center of the Johannes Gutenberg University-
Autor(es): dc.contributorUniversity of Bern-
Autor(es): dc.creatorGutierrez, Lorena S.-
Autor(es): dc.creatorZandim-Barcelos, Daniela L.-
Autor(es): dc.creatorEick, Sigrun-
Autor(es): dc.creatorLopes, Maria Eduarda S.-
Autor(es): dc.creatorCirelli, Joni A.-
Autor(es): dc.creatorNogueira, Andressa V. B.-
Autor(es): dc.creatorDeschner, James-
Data de aceite: dc.date.accessioned2025-08-21T18:56:29Z-
Data de disponibilização: dc.date.available2025-08-21T18:56:29Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s00784-024-06043-0-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/297953-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/297953-
Descrição: dc.descriptionObjectives: The present study aimed to investigate a possible immunomodulatory role of the periodontopathogen Filifactor alocis through the antimicrobial peptide hBD-2 on the expression of chemokines in human gingival keratinocytes. Materials and methods: Cells were cultured in the presence or absence of periodontopathogenic bacteria, such as F. alocis, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and Treponema denticola, to evaluate the regulation of hBD-2, CXCL8 and CCL20. Furthermore, the cells were exposed or not to hBD-2 and the expression of CXCL8 and CCL20 and their receptors was evaluated. Results: All bacteria induced a significant upregulation of hBD-2, CXCL8, and CCL20 gene expressions. In addition, F. alocis significantly increased their protein levels, as detected by ELISA. Pre-incubation of the cells with the TLR2 inhibitor resulted in a significant downregulation of hBD-2 expression in F. alocis-treated cells. Gingival keratinocytes exposed to hBD-2 resulted in a significant and dose-dependent increase of all chemokines and their receptors. Conclusions: F. alocis increased the production of chemotactic cytokines, suggesting an increase in the recruitment of immunoinflammatory cells in periodontal diseases. The chemotaxis-promoting effect is partly direct, but is also mediated via hBD-2. F. alocis stimulates the synthesis of hBD-2, which in turn could promote the expression and synthesis of these chemokines and their receptors. In addition, hBD-2 has an autostimulatory effect and stimulates the synthesis of these chemokines, so that the chemotaxis triggered by F. alocis is further fueled. Clinical relevance: F. alocis and hBD-2 have a significant role in periodontitis, showing their importance for diagnostic and treatment approaches.-
Descrição: dc.descriptionDeutscher Akademischer Austauschdienst-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery School of Dentistry at Araraquara São Paulo State University-UNESP, SP-
Descrição: dc.descriptionDepartment of Periodontology and Operative Dentistry University Medical Center of the Johannes Gutenberg University-
Descrição: dc.descriptionLaboratory of Oral Microbiology Department of Periodontology University of Bern-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery School of Dentistry at Araraquara São Paulo State University-UNESP, SP-
Descrição: dc.descriptionDeutscher Akademischer Austauschdienst: 57391253-
Descrição: dc.descriptionCAPES: 88881.144012/2017-01-
Idioma: dc.languageen-
Relação: dc.relationClinical Oral Investigations-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBeta-defensins-
Palavras-chave: dc.subjectEpithelial cells-
Palavras-chave: dc.subjectImmune response-
Palavras-chave: dc.subjectInflammation-
Palavras-chave: dc.subjectPeriodontitis-
Título: dc.titlePossible immunomodulatory role of Filifactor alocis through beta-defensin 2 in gingival keratinocytes-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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