Microbial adhesion and biofilm formation by Candida albicans on 3D-printed denture base resins

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorMcGill University-
Autor(es): dc.creatorSilva, Marcela Dantas Dias da-
Autor(es): dc.creatorNunes, Thais Soares Bezerra Santos-
Autor(es): dc.creatorViotto, Hamile Emanuella do Carmo-
Autor(es): dc.creatorCoelho, Sabrina Romão Gonçalves-
Autor(es): dc.creatorSouza, Raphael Freitas de-
Autor(es): dc.creatorPero, Ana Carolina-
Data de aceite: dc.date.accessioned2025-08-21T18:18:59Z-
Data de disponibilização: dc.date.available2025-08-21T18:18:59Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-10-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0292430-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/297920-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/297920-
Descrição: dc.descriptionThis study evaluated surface properties and adhesion/biofilm formation by Candida albicans on 3D printed denture base resins used in 3D printing. Disc-shaped specimens (15 mm x 3 mm) of two 3D-printed resins (NextDent Denture 3D+, NE, n = 64; and Cosmos Denture, CO, n = 64) and a heat-polymerized resin (Lucitone 550, LU, control, n = 64) were analyzed for surface roughness (Ra μm) and surface free energy (erg cm-2). Microbiologic assays (90-min adhesion and 48-h biofilm formation by C. albicans) were performed five times in triplicate, with the evaluation of the specimens’ surface for: (i) colony forming units count (CFU/mL), (ii) cellular metabolism (XTT assay), and (iii) fluorescence and thickness of biofilm layers (confocal laser scanning microscopy). Data were analyzed using parametric and nonparametric tests (α = 0.05). LU presented higher surface roughness Ra (0.329 ±0.076 μm) than NE (0.295±0.056 μm) (p = 0.024), but both were similar to CO (0.315 ±0.058 μm) (p = 1.000 and p = 0.129, respectively). LU showed lower surface free energy (47.47±2.01 erg cm-2) than CO (49.61±1.88 erg cm-2) and NE (49.23±2.16 erg cm-2) (p<0.001 for both). The CO and NE resins showed greater cellular metabolism (p<0.001) and CO only, showed greater colonization (p = 0.015) by C. albicans than LU in the 90-min and 48-hour periods. It can be concluded that both 3D-printed denture base resins are more prone to colonization by C. albicans, and that their surface free energy may be more likely associated with that colonization than their surface roughness.-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics Araraquara School of Dentistry São Paulo State University (UNESP), SP-
Descrição: dc.descriptionFaculty of Dental Medicine and Oral Health Sciences McGill University-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics Araraquara School of Dentistry São Paulo State University (UNESP), SP-
Idioma: dc.languageen-
Relação: dc.relationPLoS ONE-
???dc.source???: dc.sourceScopus-
Título: dc.titleMicrobial adhesion and biofilm formation by Candida albicans on 3D-printed denture base resins-
Tipo de arquivo: dc.typelivro digital-
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