Signal Amplification for Cell-Free Biosensors, an Analog-to-Digital Converter

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)-
Autor(es): dc.contributorUniversidade Federal do ABC (UFABC)-
Autor(es): dc.creatorFranco, Rafael A. L.-
Autor(es): dc.creatorBrenner, Gabriel-
Autor(es): dc.creatorZocca, Vitória F. B.-
Autor(es): dc.creatorde Paiva, Gabriela B.-
Autor(es): dc.creatorLima, Rayane N.-
Autor(es): dc.creatorRech, Elibio L.-
Autor(es): dc.creatorAmaral, Danilo T.-
Autor(es): dc.creatorLins, Milca R. C. R.-
Autor(es): dc.creatorPedrolli, Danielle B.-
Data de aceite: dc.date.accessioned2025-08-21T19:24:30Z-
Data de disponibilização: dc.date.available2025-08-21T19:24:30Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-10-19-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1021/acssynbio.3c00227-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/297909-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/297909-
Descrição: dc.descriptionToehold switches are biosensors useful for the detection of endogenous and environmental RNAs. They have been successfully engineered to detect virus RNAs in cell-free gene expression reactions. Their inherent sequence programmability makes engineering a fast and predictable process. Despite improvements in the design, toehold switches suffer from leaky translation in the OFF state, which compromises the fold change and sensitivity of the biosensor. To address this, we constructed and tested signal amplification circuits for three toehold switches triggered by Dengue and SARS-CoV-2 RNAs and an artificial RNA. The serine integrase circuit efficiently contained leakage, boosted the expression fold change from OFF to ON, and decreased the detection limit of the switches by 3-4 orders of magnitude. Ultimately, the integrase circuit converted the analog switches’ signals into digital-like output. The circuit is broadly useful for biosensors and eliminates the hard work of designing and testing multiple switches to find the best possible performer.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionUniversidade Estadual Paulista (UNESP) School of Pharmaceutical Sciences Department of Bioprocess Engineering and Biotechnology, Rodovia Araraquara-Jau km1-
Descrição: dc.descriptionEmbrapa Genetic Resources and Biotechnology National Institute of Science and Technology - Synthetic Biology-
Descrição: dc.descriptionFederal University of ABC (UFABC) Center for Natural and Human Sciences, Avenida dos Estados 5001-
Descrição: dc.descriptionUniversidade Estadual Paulista (UNESP) School of Pharmaceutical Sciences Department of Bioprocess Engineering and Biotechnology, Rodovia Araraquara-Jau km1-
Formato: dc.format2819-2826-
Idioma: dc.languageen-
Relação: dc.relationACS Synthetic Biology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectbiosensor-
Palavras-chave: dc.subjectcell-free system-
Palavras-chave: dc.subjectDengue-
Palavras-chave: dc.subjectSARS-CoV-2-
Palavras-chave: dc.subjectserine integrase-
Palavras-chave: dc.subjecttoehold switch-
Título: dc.titleSignal Amplification for Cell-Free Biosensors, an Analog-to-Digital Converter-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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