Antimicrobial Silk Fibroin Methacrylated Scaffolds for Regenerative Endodontics

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversity of Michigan–School of Dentistry-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Federal de Minas Gerais (UFMG)-
Autor(es): dc.contributorStony Brook University–School of Dental Medicine-
Autor(es): dc.contributorUniversity of Michigan-
Autor(es): dc.creatorNarayanam, Ramyasaketha-
Autor(es): dc.creatorCardoso, Lais M.-
Autor(es): dc.creatordos Reis-Prado, Alexandre H.-
Autor(es): dc.creatorde Carvalho, Ana Beatriz G.-
Autor(es): dc.creatorAnselmi, Caroline-
Autor(es): dc.creatorMahmoud, Abdel H.-
Autor(es): dc.creatorFenno, J. Christopher-
Autor(es): dc.creatorDal-Fabbro, Renan-
Autor(es): dc.creatorBottino, Marco C.-
Data de aceite: dc.date.accessioned2025-08-21T16:46:12Z-
Data de disponibilização: dc.date.available2025-08-21T16:46:12Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.joen.2024.08.004-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/297799-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/297799-
Descrição: dc.descriptionIntroduction: Recognizing the necessity of novel disinfection strategies for improved bacterial control to ultimately favor tissue regeneration, this study developed and characterized antibiotics-laden silk fibroin methacrylated (SilkMA) scaffolds for regenerative endodontics. Methods: SilkMA-based solutions (10% w/v) containing Clindamycin (CLI) or Tinidazole (TIN) (0 – control; 5, 10, or 15% w/w) or the combination of both drugs (BiMix CLI/TIN 10%) were electrospun and photocrosslinked. Morphology and composition were assessed using scanning electron microscopy and Fourier-transform infrared spectroscopy. Additionally, swelling and degradation profiles were also determined. Cytotoxicity was evaluated in stem cells from apical papilla. Antibacterial efficacy was tested using direct and indirect contact assays against Aggregatibacter actinomycetemcomitans/Aa, Actinomyces naeslundii/An, Enterococcus faecalis/Ef, and Fusobacterium nucleatum/Fn. E. faecalis biofilm inhibition on dentin discs was specifically evaluated for BiMix-laden scaffolds. Data were statistically analyzed with a significance level of 5%. Results: Scanning electron microscopy revealed that all scaffolds had similar characteristics, including fiber morphology and bead absence. Fourier-transform infrared spectroscopy showed the incorporation of CLI and TIN into the fibers and in BiMix scaffolds. Antibiotic-laden scaffolds exhibited lower swelling capacity than the control and were degraded entirely after 45 days. Scaffolds laden with CLI, TIN, or BiMix throughout all time points did not reduce stem cells from apical papilla's viability. CLI-laden scaffolds inhibited the growth of Aa, An, and Ef, while TIN-laden scaffolds inhibited Fn growth. BiMix-laden scaffolds significantly inhibited Aa, An, Ef, and Fn in direct contact, and their aliquots inhibited An and Fn through indirect contact, with additional biofilm inhibition against Ef. Conclusions: BiMix-laden SilkMA scaffolds are cytocompatible and exhibit antimicrobial effects against endodontic pathogens, indicating their therapeutic potential as a drug delivery system for regenerative endodontics.-
Descrição: dc.descriptionDepartment of Cariology Restorative Sciences and Endodontics University of Michigan–School of Dentistry-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics São Paulo State University (UNESP)–Araraquara School of Dentistry, São Paulo-
Descrição: dc.descriptionDepartment of Restorative Dentistry Minas Gerais Federal University (UFMG)–School of Dentistry, Minas Gerais-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics São Paulo State University (UNESP)–Sao Jose dos Campos School of Dentistry, São Paulo-
Descrição: dc.descriptionDepartment of Morphology and Pediatric Dentistry São Paulo State University (UNESP)–Araraquara School of Dentistry, São Paulo-
Descrição: dc.descriptionDepartment of Oral Biology and Pathology Stony Brook University–School of Dental Medicine-
Descrição: dc.descriptionDepartment of Biologic and Materials Sciences & Prosthodontics University of Michigan–School of Dentistry-
Descrição: dc.descriptionDepartment of Biomedical Engineering College of Engineering University of Michigan-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics São Paulo State University (UNESP)–Araraquara School of Dentistry, São Paulo-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics São Paulo State University (UNESP)–Sao Jose dos Campos School of Dentistry, São Paulo-
Descrição: dc.descriptionDepartment of Morphology and Pediatric Dentistry São Paulo State University (UNESP)–Araraquara School of Dentistry, São Paulo-
Formato: dc.format1752-1760.e2-
Idioma: dc.languageen-
Relação: dc.relationJournal of Endodontics-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectClindamycin-
Palavras-chave: dc.subjectregenerative endodontics-
Palavras-chave: dc.subjectsilk-
Palavras-chave: dc.subjecttinidazole-
Palavras-chave: dc.subjecttissue engineering-
Título: dc.titleAntimicrobial Silk Fibroin Methacrylated Scaffolds for Regenerative Endodontics-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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