Injectable progesterone in 13-month-old prepubertal Nellore heifers: effects on in vitro embryo production and pregnancy rate

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorBeef Cattle Research Center-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorVitrogen-WTAvet-
Autor(es): dc.contributorEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)-
Autor(es): dc.creatorda Silva, Letícia Padovani-
Autor(es): dc.creatorde Oliveira Silva, Marina-
Autor(es): dc.creatorBorges, Marcelo Sant’Ana-
Autor(es): dc.creatorPagoto, Jaine Martelo-
Autor(es): dc.creatorWatanabe, Yeda Fumie-
Autor(es): dc.creatorOliveira, Clara Slade-
Autor(es): dc.creatorZerlotti Mercadante, Maria Eugênia-
Autor(es): dc.creatorMonteiro, Fabio Morato-
Data de aceite: dc.date.accessioned2025-08-21T17:35:51Z-
Data de disponibilização: dc.date.available2025-08-21T17:35:51Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-10-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s11250-024-04205-6-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/297777-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/297777-
Descrição: dc.descriptionThe aim of this study was to evaluate the effect of injectable progesterone administration before ovum pick-up (OPU) on in vitro embryo production (IVEP) and pregnancy rate in prepubertal Nellore heifers. Twenty-three Nellore females, 13.04 ± 0.8 months of age, were randomly assigned to two groups: control group (no progesterone application) and progesterone group (intramuscular administration of 1 mL [150 mg/mL] progesterone 7 days before OPU). The second OPU was performed after 28 days (crossover between groups). After OPU, oocytes were selected for IVEP and a sample was fixed in 4% paraformaldehyde. Viable oocytes were subjected to immunofluorescence staining for IGFBP2 and caspase-3. For each oocyte, one image was generated per channel (A555 and A488 filters) and analyzed with the ImageJ program for the quantification of fluorescence intensity (in pixels). The produced embryos were transferred to multiparous Nellore cows and pregnancy was diagnosed by ultrasound 30 days after transfer. Data were analyzed using the MIXED and GLIMMIX procedure of SAS®. Treatment with injectable progesterone had no effect on the number or size of the antral follicles of heifers. No differences between the control and progesterone groups were observed for total number of aspirated oocytes (P = 0.78), viable oocytes (P = 0.80), cleavage rate (P = 0.60), or blastocyst rate (P = 0.56). There were no differences in pixel intensity between the two groups, nor in the levels of IGFBP2 or caspase-3. However, a difference was observed in the pregnancy rate of embryos produced from heifers of the progesterone group (42.1%; 32/76) when compared to the rate of embryos produced from control heifers (28.4%; 23/81) (P = 0.02). The use of injectable progesterone before OPU in prepubertal Nellore heifers does not improve IVEP but increases the pregnancy rate after transfer.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionInstitute of Animal Science Beef Cattle Research Center, SP-
Descrição: dc.descriptionSão Paulo State University (UNESP) Faculty of Agricultural and Veterinarian Sciences, SP-
Descrição: dc.descriptionVitrogen-WTAvet, SP-
Descrição: dc.descriptionEmbrapa Dairy Cattle Gado de Leite Animal Reproduction Laboratory, RJ-
Descrição: dc.descriptionSão Paulo State University (UNESP) Faculty of Agricultural and Veterinarian Sciences, SP-
Descrição: dc.descriptionCAPES: 001-
Descrição: dc.descriptionFAPESP: 2017/50339-5-
Idioma: dc.languageen-
Relação: dc.relationTropical Animal Health and Production-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAnimal reproduction-
Palavras-chave: dc.subjectFollicular aspiration-
Palavras-chave: dc.subjectIVEP-
Título: dc.titleInjectable progesterone in 13-month-old prepubertal Nellore heifers: effects on in vitro embryo production and pregnancy rate-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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