Differential Modulation of Saliva-Derived Microcosm Biofilms by Antimicrobial Peptide LL-31 and D-LL-31

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Autor(es): dc.contributorUniversity of Amsterdam and Vrije Universiteit Amsterdam-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorSichuan University-
Autor(es): dc.creatorSoldati, Kahena R.-
Autor(es): dc.creatorJiang, Yaling-
Autor(es): dc.creatorBrandt, Bernd W.-
Autor(es): dc.creatorExterkate, Rob A. M.-
Autor(es): dc.creatorBuijs, Mark J.-
Autor(es): dc.creatorNazmi, Kamran-
Autor(es): dc.creatorKaman, Wendy E.-
Autor(es): dc.creatorCheng, Lei-
Autor(es): dc.creatorBikker, Floris J.-
Autor(es): dc.creatorCrielaard, Wim-
Autor(es): dc.creatorZandim-Barcelos, Daniela L.-
Autor(es): dc.creatorDeng, Dong Mei-
Data de aceite: dc.date.accessioned2025-08-21T18:12:40Z-
Data de disponibilização: dc.date.available2025-08-21T18:12:40Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-10-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/pathogens12111295-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/297702-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/297702-
Descrição: dc.descriptionMicrobiome modulation, aiming to restore a health-compatible microbiota, is a novel strategy to treat periodontitis. This study evaluated the modulation effects of antimicrobial peptide LL-31 and its D-enantiomer (D-LL-31) on saliva-derived microcosm biofilms, spiked with or without Porphyromonas gingivalis. To this end, one-day-old biofilms were incubated for 24 h with biofilm medium alone, or medium containing 40 µM LL-31 or D-LL-31, after which biofilms were grown for 5 days. Biofilms were assessed at 1 day and 5 days after intervention for the total viable cell counts, dipeptidyl peptidase IV (DPP4) activity, P. gingivalis amount (by qPCR) and microbial composition (by sequencing). The results showed that D-LL-31, not LL-31, significantly reduced the total viable cell counts, the P. gingivalis amount, and the DPP4 activity of the biofilms spiked with P. gingivalis, but only at 1 day after intervention. In the biofilms spiked with P. gingivalis, D-LL-31 tended to reduce the α-diversity and the compositional shift of the biofilms in time as compared to the control and LL-31 groups. In conclusion, D-LL-31 showed a better performance than LL-31 in biofilm modulation. The biofilm modulation function of the peptides could be impaired when the biofilms were in a severely dysbiotic state.-
Descrição: dc.descriptionDepartment of Preventive Dentistry Academic Center for Dentistry Amsterdam (ACTA) University of Amsterdam and Vrije Universiteit Amsterdam, LA-
Descrição: dc.descriptionDepartment of Oral Diagnosis and Surgery School of Dentistry at Araraquara Universidade Estadual Paulista—UNESP, SP-
Descrição: dc.descriptionState Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases & Department of Operative Dentistry and Endodontics West China Hospital of Stomatology Sichuan University-
Descrição: dc.descriptionDepartment of Oral Biochemistry Academic Centre for Dentistry Amsterdam (ACTA) University of Amsterdam and Vrije Universiteit Amsterdam-
Descrição: dc.descriptionDepartment of Oral Diagnosis and Surgery School of Dentistry at Araraquara Universidade Estadual Paulista—UNESP, SP-
Idioma: dc.languageen-
Relação: dc.relationPathogens-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subject16S rRNA gene amplicon sequencing-
Palavras-chave: dc.subjectoral microbiome-
Palavras-chave: dc.subjectperiodontitis-
Palavras-chave: dc.subjectPorphyromonas gingivalis-
Palavras-chave: dc.subjectsaliva-derived microcosm biofilms-
Título: dc.titleDifferential Modulation of Saliva-Derived Microcosm Biofilms by Antimicrobial Peptide LL-31 and D-LL-31-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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