Chronic exposure to lipopolysaccharides as an in vitro model to simulate the impaired odontogenic potential of dental pulp cells under pulpitis conditions

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorSchool of Dentistry-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorMendes Soares, Igor Paulino-
Autor(es): dc.creatorAnselmi, Caroline-
Autor(es): dc.creatorPires, Maria Luiza Barucci Araujo-
Autor(es): dc.creatorRibeiro, Rafael Antonio de Oliveira-
Autor(es): dc.creatorLeite, Maria Luísa-
Autor(es): dc.creatorSoares, Diana Gabriela-
Autor(es): dc.creatorde Souza Costa, Carlos Alberto-
Autor(es): dc.creatorHebling, Josimeri-
Data de aceite: dc.date.accessioned2025-08-21T20:04:51Z-
Data de disponibilização: dc.date.available2025-08-21T20:04:51Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2022-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1590/1678-7757-2023-0032-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/297678-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/297678-
Descrição: dc.descriptionSimulating a bacterial-induced pulpitis environment in vitro may contribute to exploring mechanisms and bioactive molecules to counteract these adverse effects. Objective: To investigate the chronic exposure of human dental pulp cells (HDPCs) to lipopolysaccharides (LPS) aiming to establish a cell culture protocol to simulate the impaired odontogenic potential under pulpitis conditions. Methodology: HDPCs were isolated from four healthy molars of different donors and seeded in culture plates in a growth medium. After 24 h, the medium was changed to an odontogenic differentiation medium (DM) supplemented or not with E. coli LPS (0-control, 0.1, 1, or 10 µg/ mL) (n=8). The medium was renewed every two days for up to seven days, then replaced with LPS-free DM for up to 21 days. The activation of NF-κB and F-actin expression were assessed (immunofluorescence) after one and seven days. On day 7, cells were evaluated for both the gene expression (RT-qPCR) of odontogenic markers (COL1A1, ALPL, DSPP, and DMP1) and cytokines (TNF, IL1B, IL8, and IL6) and the production of reactive nitrogen (Griess) and oxygen species (Carboxy-H2DCFDA). Cell viability (alamarBlue) was evaluated weekly, and mineralization was assessed (Alizarin Red) at 14 and 21 days. Data were analyzed with ANOVA and post-hoc tests (α=5%). Results: After one and seven days of exposure to LPS, NF-κB was activated in a dose-dependent fashion. LPS at 1 and 10 µg/mL concentrations down-regulated the gene expression of odontogenic markers and up-regulated cytokines. LPS at 10 µg/mL increased both the production of reactive nitrogen and oxygen species. LPS decreased cell viability seven days after the end of exposure. LPS at 1 and 10 µg/mL decreased hDPCs mineralization in a dose-dependent fashion. Conclusion: The exposure to 10 µg/mL LPS for seven days creates an inflammatory environment that is able to impair by more than half the odontogenic potential of HDPCs in vitro, simulating a pulpitis-like condition.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionUniversidade Estadual Paulista-UNESP Faculdade de Odontologia de Araraquara Departamento de Materiais Odontológicos e Prótese, SP-
Descrição: dc.descriptionUniversidade Estadual Paulista-UNESP Faculdade de Odontologia de Araraquara Departamento de Morfologia e Clínica Infantil, SP-
Descrição: dc.descriptionDepartment of Oral Health Sciences The University of British Columbia School of Dentistry-
Descrição: dc.descriptionUniversidade de São Paulo Faculdade de Odontologia de Bauru Departamento de Dentística Endodontia e Materiais Odontológicos, SP-
Descrição: dc.descriptionUniversidade Estadual Paulista-UNESP Faculdade de Odontologia de Araraquara Departamento de Fisiologia e Patologia, SP-
Descrição: dc.descriptionUniversidade Estadual Paulista-UNESP Faculdade de Odontologia de Araraquara Departamento de Materiais Odontológicos e Prótese, SP-
Descrição: dc.descriptionUniversidade Estadual Paulista-UNESP Faculdade de Odontologia de Araraquara Departamento de Morfologia e Clínica Infantil, SP-
Descrição: dc.descriptionUniversidade Estadual Paulista-UNESP Faculdade de Odontologia de Araraquara Departamento de Fisiologia e Patologia, SP-
Descrição: dc.descriptionFAPESP: 2019/16473-1-
Descrição: dc.descriptionFAPESP: 2021/13096-2-
Descrição: dc.descriptionCNPq: 303391/2019-7-
Idioma: dc.languageen-
Relação: dc.relationJournal of Applied Oral Science-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBiomineralization-
Palavras-chave: dc.subjectCell Culture Techniques-
Palavras-chave: dc.subjectDental Pulp-
Palavras-chave: dc.subjectLipopolysaccharides-
Palavras-chave: dc.subjectPulpitis-
Título: dc.titleChronic exposure to lipopolysaccharides as an in vitro model to simulate the impaired odontogenic potential of dental pulp cells under pulpitis conditions-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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