Salmonella spp. detection in fresh and freeze-dried frog meat: a comparison of methods

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorand Post Graduate Program in Food and Nutrition Security at the Federal University of the State of Rio de Janeiro (Unirio)-
Autor(es): dc.contributorState Center for Animal Health Research of the Agricultural Research Corporation of the State of Rio de Janeiro (Pesagro)-
Autor(es): dc.contributorState Center for Food Quality Research of the Agricultural Research Corporation of the State of Rio de Janeiro (Pesagro)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorLaboratory of Fish and Aquatic Animals’ Health of the Veterinary Faculty-
Autor(es): dc.creatorMello, Silvia Conceição Reis Pereira-
Autor(es): dc.creatorDE SOUZA, Rossiane M.-
Autor(es): dc.creatorRodrigues, Eliane-
Autor(es): dc.creatorCaracuschansk, Fernando David-
Autor(es): dc.creatorMendes, Beatriz V.-
Autor(es): dc.creatorDE MESQUITA, ELIANA DE FÁTIMA M.-
Data de aceite: dc.date.accessioned2025-08-21T22:43:17Z-
Data de disponibilização: dc.date.available2025-08-21T22:43:17Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1590/0001-3765202520230552-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/297677-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/297677-
Descrição: dc.descriptionThis study examined the presence of Salmonella spp. in fresh frog meat and freeze-dried seasoned frog meat stored for up to 90 days at room temperature. The samples were analyzed in triplicate using the conventional official method, by polymerase chain reaction (PCR) and mass spectrometry technique (MALDI –TOF) — for a comparison of the results obtained by the three tested methods. Mass spectrometry analyses confirmed the presence of Salmonella spp. in 100% of the fresh frog meat samples. In the analysis by the conventional method, the percentages of detection were 67% and 100% in samples B2 and B3, whereas the PCR method indicated 100% and 67% presence of the bacteria in the respective samples. In the analyses of freeze-dried none of the three methods detected the presence of Salmonella spp. The different analysis methods tested in this study were efficient in determining the presence or absence of Salmonella spp. in the samples, and the comparison between methods revealed a high percentage of compatibility of results. Adoption of efficient detection methods, such as PCR and MALDI-TOF, is essential, not only for public health, but also for compliance with Brazilian regulations, ensuring that food products are free from Salmonella spp. contamination and safe for consumption.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionState Center for Food Quality Research of the Agricultural Research Corporation of the State of Rio de Janeiro (Pesagro) Fisheries Institute Foundation of the State of Rio de Janeiro (Fiperj) and Post Graduate Program in Food and Nutrition Security at the Federal University of the State of Rio de Janeiro (Unirio), Alameda São Boa Ventura, 770, Fonseca, RJ-
Descrição: dc.descriptionState Center for Animal Health Research of the Agricultural Research Corporation of the State of Rio de Janeiro (Pesagro), Alameda São Boa Ventura, 770, Fonseca, RJ-
Descrição: dc.descriptionState Center for Food Quality Research of the Agricultural Research Corporation of the State of Rio de Janeiro (Pesagro), Alameda São Boa Ventura, 770, Fonseca, RJ-
Descrição: dc.descriptionSão Paulo State University Júlio de Mesquita Filho (FCAV/ Unesp), Via de Acesso Prof. Paulo Donato Castelane, s/n, Vila Industrial, SP-
Descrição: dc.descriptionFluminense Federal University (UFF) Laboratory of Fish and Aquatic Animals’ Health of the Veterinary Faculty, Rua Vital Brasil Filho, 64, Vital Brasil, RJ-
Descrição: dc.descriptionSão Paulo State University Júlio de Mesquita Filho (FCAV/ Unesp), Via de Acesso Prof. Paulo Donato Castelane, s/n, Vila Industrial, SP-
Descrição: dc.descriptionFAPERJ: 262234/2021-
Descrição: dc.descriptionCNPq: DT-313302/2019-7-
Descrição: dc.descriptionFAPERJ: E-26/010.001586/2019-
Idioma: dc.languageen-
Relação: dc.relationAnais da Academia Brasileira de Ciencias-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAquarana catesbeiana-
Palavras-chave: dc.subjectMALDI-TOF-
Palavras-chave: dc.subjectmicrobiological analysis of foods-
Palavras-chave: dc.subjectpoly-merase chain reaction-
Título: dc.titleSalmonella spp. detection in fresh and freeze-dried frog meat: a comparison of methods-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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