Enhancing Antifungal Treatment of Candida albicans with Hypericin-Loaded Nanostructured Lipid Carriers in Hydrogels: Characterization, In Vitro, and In Vivo Photodynamic Evaluation

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorState University of Paraíba-
Autor(es): dc.contributorUniversity of Ulster-
Autor(es): dc.creatorSato, Mariana Rillo-
Autor(es): dc.creatorOshiro-Junior, João Augusto-
Autor(es): dc.creatorRodero, Camila Fernanda-
Autor(es): dc.creatorBoni, Fernanda Isadora-
Autor(es): dc.creatorAraújo, Victor Hugo Sousa-
Autor(es): dc.creatorBauab, Taís Maria-
Autor(es): dc.creatorNicholas, Dean-
Autor(es): dc.creatorCallan, John Francis-
Autor(es): dc.creatorChorilli, Marlus-
Data de aceite: dc.date.accessioned2025-08-21T20:26:13Z-
Data de disponibilização: dc.date.available2025-08-21T20:26:13Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-08-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/ph16081094-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/297548-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/297548-
Descrição: dc.descriptionBackground: Vulvovaginal candidiasis (VVC) is a worldwide public health problem caused predominantly by the opportunistic polymorphic fungus Candida albicans, whose pathogenicity is associated with its morphological adaptability. To potentiate the treatment of C. albicans-induced VVC by an alternative method as photodynamic therapy (PDT), hypericin (Hy), a potent photosensitizer compound was incorporated into a nanostructured lipid carrier (NLC) and dispersed in hydrogel (HG). Methods: After preparation of the sonication process, an NLC loaded with Hy was dispersed in HG based on Poloxamer 407 and chitosan obtaining Hy.NLC-HG. This hydrogel system was physically and chemically characterized and its in vitro and in vivo photodynamic and antifungal effects were evaluated. Results: Through scanning electron microscopy, it was possible to observe a hydrogel system with a porous polymeric matrix and irregular microcavities. The Hy.NLC-HG system showed mucoadhesive properties (0.45 ± 0.08 N) and a satisfactory injectability (15.74 ± 4.75 N.mm), which indicates that it can be easily applied in the vaginal canal, in addition to a controlled and sustained Hy release profile from the NLC-HG of 28.55 ± 0.15% after 720 min. The in vitro antibiofilm assay significantly reduced the viability of C. albicans (p < 0.001) by 1.2 log10 for Hy.NLC-HG/PDT and 1.9 log10 for PS/PDT, Hy.NLC/PDT, and free RB/PDT, compared to the PBS/PDT negative control. The in vivo antifungal evaluation showed that animals treated with the vaginal cream (non-PDT) and the PDT-mediated Hy.NLC-HG system showed a significant difference of p < 0.001 in the number of C. albicans colonies (log) in the vaginal canal, compared to the inoculation control group. Conclusions: Thus, we demonstrate the pharmaceutical, antifungal, and photodynamic potential of hydrogel systems for Hy vaginal administration.-
Descrição: dc.descriptionUlster University-
Descrição: dc.descriptionSchool of Pharmaceutical Sciences São Paulo State University (UNESP), SP-
Descrição: dc.descriptionGraduation Program in Pharmaceutical Sciences State University of Paraíba, PB-
Descrição: dc.descriptionBiomedical Sciences Research Institute University of Ulster-
Descrição: dc.descriptionSchool of Pharmaceutical Sciences São Paulo State University (UNESP), SP-
Idioma: dc.languageen-
Relação: dc.relationPharmaceuticals-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectantifungal agent-
Palavras-chave: dc.subjectmucoadhesiveness-
Palavras-chave: dc.subjectnanostructured system-
Palavras-chave: dc.subjectphotosensitizer-
Palavras-chave: dc.subjectyeast-
Título: dc.titleEnhancing Antifungal Treatment of Candida albicans with Hypericin-Loaded Nanostructured Lipid Carriers in Hydrogels: Characterization, In Vitro, and In Vivo Photodynamic Evaluation-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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