Guttiferone E from Brazilian red propolis inhibited wound-isolated methicillin-resistant Staphylococcus aureus and enhanced the bactericidal action of suppressed macrophages

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorCenter for Studies of Biomolecules Applied to Health (CEBio)-
Autor(es): dc.contributorOswaldo Cruz Foundation (LABIOPROT/ FIOCRUZ)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorRipari, Nicolas-
Autor(es): dc.creatorLopes, Emilly Camargo-
Autor(es): dc.creatorFrancisco, Áleff Ferreira-
Autor(es): dc.creatorde Almeida, Jônatas Felipe Santos-
Autor(es): dc.creatorHonorio, Mariana da Silva-
Autor(es): dc.creatorJúnior, Ary Fernandes-
Autor(es): dc.creatorFontes, Marcos Roberto de Mattos-
Autor(es): dc.creatorTanimoto, Matheus Hikaru-
Autor(es): dc.creatorCalderon, Leonardo de Azevedo-
Autor(es): dc.creatorBastos, Jairo Kenupp-
Autor(es): dc.creatorSforcin, José Maurício-
Data de aceite: dc.date.accessioned2025-08-21T17:29:27Z-
Data de disponibilização: dc.date.available2025-08-21T17:29:27Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2025-05-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.phymed.2025.156615-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/297480-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/297480-
Descrição: dc.descriptionBackground: Propolis has been traditionally used to treat inflammatory and infectious diseases, and it is still used and researched worldwide. Methicillin-resistant Staphylococcus aureus (MRSA) may cause invasive infections and propolis anti-MRSA activity has been analyzed. Purpose: A standardized red propolis extract (SRPE), its benzophenones-rich fraction (BRF), and isolated benzophenones (guttiferone E - GUT E, and oblongifolin B - OBL B) were assayed for their antibacterial and immunomodulatory action. Methods: Formulations (BRP28, BRP29, BRP150, BRP153, and BRPLUS) were prepared and their minimum inhibitory concentrations (MIC) were assessed. The synergistic action of GUT E with antimicrobials was evaluated on a wound-isolated MRSA, as well as the inhibition of biofilm formation by the formulations (BRP28 and BRP29) and GUT E. Tohoku Hospital Pediatrics-1 (THP-1) cells were used to investigate cytokine production and the bactericidal activity of suppressed macrophages against MRSA. Computational predictions were performed with GUT E and antimicrobials to observe their interaction with the active and allosteric site of penicillin-binding protein 2a (PBP2a). Results: SRPE and BRF were not efficient against MRSA while GUT E and OBL B exerted a potent activity. GUT E exerted a synergistic effect with carbapenems and vancomycin. BRP28, BRP29, and GUT E inhibited biofilm formation and increased the antibacterial capacity of suppressed macrophages, with no differences in cytokine production. GUT E showed a high binding affinity to PBP2a. Conclusion: GUT E exhibited a direct anti-MRSA activity and indirectly enhanced the macrophage bactericidal activity. Molecular docking suggested that GUT E has a versatile interaction with PBP2a.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionSão Paulo State University (UNESP) Institute of Biosciences, Campus Botucatu-
Descrição: dc.descriptionOswaldo Cruz Foundation (Fiocruz) Fiocruz Rondônia Center for Studies of Biomolecules Applied to Health (CEBio), Rondônia-
Descrição: dc.descriptionLaboratory of Biotechnology of Proteins and Bioactive Components Oswaldo Cruz Foundation (LABIOPROT/ FIOCRUZ), Rondônia-
Descrição: dc.descriptionSão Paulo State University (UNESP) Institute for Advanced Studies of the Sea (IEAMar), Campus São Vicente-
Descrição: dc.descriptionUniversity of São Paulo (USP) School of Pharmaceutical Sciences of Ribeirão Preto, Campus Ribeirão Preto-
Descrição: dc.descriptionSão Paulo State University (UNESP) Institute of Biosciences, Campus Botucatu-
Descrição: dc.descriptionSão Paulo State University (UNESP) Institute for Advanced Studies of the Sea (IEAMar), Campus São Vicente-
Descrição: dc.descriptionFAPESP: 2017/04138-8-
Descrição: dc.descriptionFAPESP: 2023/01436-9-
Idioma: dc.languageen-
Relação: dc.relationPhytomedicine-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBioinformatics-
Palavras-chave: dc.subjectBrazilian red propolis-
Palavras-chave: dc.subjectGuttiferone E-
Palavras-chave: dc.subjectMacrophage-
Palavras-chave: dc.subjectMultidrug-resistant bacteria-
Título: dc.titleGuttiferone E from Brazilian red propolis inhibited wound-isolated methicillin-resistant Staphylococcus aureus and enhanced the bactericidal action of suppressed macrophages-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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