Improved biosensing of Legionella by integrating filtration and immunomagnetic separation of the bacteria retained in filters

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Autor(es): dc.contributorUniversitat Autònoma de Barcelona-
Autor(es): dc.contributorInstitut d’Investigació Germans Trias i Pujol (IGTP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorMesas Gómez, Melania-
Autor(es): dc.creatorMolina-Moya, Bárbara-
Autor(es): dc.creatorde Araujo Souza, Bárbara-
Autor(es): dc.creatorBoldrin Zanoni, Maria Valnice-
Autor(es): dc.creatorJulián, Esther-
Autor(es): dc.creatorDomínguez, José-
Autor(es): dc.creatorPividori, Maria Isabel-
Data de aceite: dc.date.accessioned2025-08-21T23:04:20Z-
Data de disponibilização: dc.date.available2025-08-21T23:04:20Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-01-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s00604-023-06122-1-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/297386-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/297386-
Descrição: dc.descriptionA novel approach is presented that combines filtration and the direct immunomagnetic separation of the retained bacteria Legionella in filters, for further electrochemical immunosensing. This strategy allows for the separation and preconcentration of the water-borne pathogen from high-volume samples, up to 1000 mL. The limit of detection of the electrochemical immunosensor resulted in 100 CFU mL−1 and improved up to 0.1 CFU mL−1 when the preconcentration strategy was applied in 1 L of sample (103-fold improvement). Remarkably, the immunosensor achieves the limit of detection in less than 2.5 h and simplified the analytical procedure. This represents the lowest concentration reported to date for electrochemical immunosensing of Legionella cells without the need for pre-enrichment or DNA amplification. Furthermore, the study successfully demonstrates the extraction of bacteria retained on different filtering materials using immunomagnetic separation, highlighting the high efficiency of the magnetic particles to pull out the bacteria directly from solid materials. This promising feature expands the applicability of the method beyond water systems for detecting bacteria retained in air filters of air conditioning units by directly performing the immunomagnetic separation in the filters. Graphical abstract: [Figure not available: see fulltext.].-
Descrição: dc.descriptionGrup de Sensors i Biosensors Departament de Química Universitat Autònoma de Barcelona-
Descrição: dc.descriptionBiosensing and Bioanalysis Group Institute of Biotechnology and Biomedicine Universitat Autònoma de Barcelona-
Descrição: dc.descriptionInstitut d’Investigació Germans Trias i Pujol (IGTP)-
Descrição: dc.descriptionCIBER Enfermedades Respiratorias Instituto de Salud Carlos III Departament de Genètica i Microbiologia Universitat Autònoma de Barcelona-
Descrição: dc.descriptionDepartment of Analytical Chemistry Institute of Chemistry UNESP Universidad Estadual Paulista, SP-
Descrição: dc.descriptionDepartament de Genètica i Microbiologia Universitat Autònoma de Barcelona-
Descrição: dc.descriptionDepartment of Analytical Chemistry Institute of Chemistry UNESP Universidad Estadual Paulista, SP-
Idioma: dc.languageen-
Relação: dc.relationMicrochimica Acta-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectElectrochemical immunosensor-
Palavras-chave: dc.subjectMagnetic particles-
Palavras-chave: dc.subjectPreconcentration strategy-
Palavras-chave: dc.subjectSolid-phase separation-
Palavras-chave: dc.subjectSquare wave voltammetry-
Palavras-chave: dc.subjectWater-borne bacteria-
Título: dc.titleImproved biosensing of Legionella by integrating filtration and immunomagnetic separation of the bacteria retained in filters-
Tipo de arquivo: dc.typelivro digital-
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