Panx1 knockout promotes preneoplastic aberrant crypt foci development in a chemically induced model of mouse colon carcinogenesis

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorEspírito Santo, Sara Gomes-
Autor(es): dc.creatorDa Silva, Tereza Cristina-
Autor(es): dc.creatorCogliati, Bruno-
Autor(es): dc.creatorBarbisan, Luís Fernando-
Autor(es): dc.creatorRomualdo, Guilherme Ribeiro-
Data de aceite: dc.date.accessioned2025-08-21T16:24:11Z-
Data de disponibilização: dc.date.available2025-08-21T16:24:11Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1111/iep.12491-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/297336-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/297336-
Descrição: dc.descriptionColorectal cancer, which is the third leading cause of cancer-related deaths worldwide, is a multistep disease, featuring preneoplastic aberrant crypt foci (ACF) as the early morphological manifestation. The roles of hemichannel-forming transmembrane Pannexin 1 (Panx1) protein have not been investigated in the context of colon carcinogenesis yet, although it has contrasting roles in other cancer types. Thus, this study was conducted to examine the effects of Panx1 knockout (Panx1−/−) on the early events of chemically induced colon carcinogenesis in mouse. Wild type (WT) and Panx1−/− female C57BL6J mice were submitted to a chemically induced model of colon carcinogenesis by receiving six intraperitoneal administrations of 1,2-dimethylhydrazine (DMH) carcinogen. Animals were euthanized 8 h (week 7) or 30 weeks (week 37) after the last DMH administration in order to evaluate sub-acute colon toxicity outcomes or the burden of ACF, respectively. At week 7, Panx1 genetic ablation increased DMH-induced genotoxicity in peripheral blood cells, malondialdehyde levels in the colon, and apoptosis (cleaved caspase-3) in colonic crypts. Of note, at week 37, Panx1−/− animals showed an increase in aberrant crypts (AC), ACF mean number, and ACF multiplicity (AC per ACF) by 56%, 57% and 20%, respectively. In essence, our findings indicate that Panx1 genetic ablation promotes preneoplastic ACF development during chemically induced mouse colon carcinogenesis, and a protective role of Panx1 is postulated.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionBotucatu Medical School Experimental Research Unit (UNIPEX) Multimodel Drug Screening Platform – Laboratory of Chemically Induced and Experimental Carcinogenesis (MDSP-LCQE) São Paulo State University (UNESP), São Paulo State-
Descrição: dc.descriptionSchool of Veterinary Medicine and Animal Science Department of Pathology University of São Paulo (USP), São Paulo State-
Descrição: dc.descriptionBiosciences Institute Department of Structural and Functional Biology São Paulo State University (UNESP), São Paulo State-
Descrição: dc.descriptionBotucatu Medical School Experimental Research Unit (UNIPEX) Multimodel Drug Screening Platform – Laboratory of Chemically Induced and Experimental Carcinogenesis (MDSP-LCQE) São Paulo State University (UNESP), São Paulo State-
Descrição: dc.descriptionBiosciences Institute Department of Structural and Functional Biology São Paulo State University (UNESP), São Paulo State-
Formato: dc.format304-312-
Idioma: dc.languageen-
Relação: dc.relationInternational Journal of Experimental Pathology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectaberrant crypt foci-
Palavras-chave: dc.subjectC57BL/6J mouse-
Palavras-chave: dc.subjectcolon carcinogenesis-
Palavras-chave: dc.subjectdimethylhydrazine-
Título: dc.titlePanx1 knockout promotes preneoplastic aberrant crypt foci development in a chemically induced model of mouse colon carcinogenesis-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.