Combination of Adult Mesenchymal Stem Cell Therapy and Immunomodulation with Dimethyl Fumarate Following Spinal Cord Ventral Root Repair

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Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorCenter for Translational Sciences and Biopharmaceuticals Development-
Autor(es): dc.creatorGelinski Kempe, Paula Regina-
Autor(es): dc.creatorde Castro, Mateus Vidigal-
Autor(es): dc.creatorCoser, Lilian de Oliveira-
Autor(es): dc.creatorCartarozzi, Luciana Politti-
Autor(es): dc.creatorBarraviera, Benedito-
Autor(es): dc.creatorFerreira, Rui Seabra-
Autor(es): dc.creatorde Oliveira, Alexandre Leite Rodrigues-
Data de aceite: dc.date.accessioned2025-08-21T15:15:07Z-
Data de disponibilização: dc.date.available2025-08-21T15:15:07Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-10-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/biology13110953-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/297251-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/297251-
Descrição: dc.descriptionSpinal cord injury results in significant motor and sensory loss. In the experimental ventral root avulsion (VRA) model, the ventral (motor) roots are disconnected from the spinal cord surface, disrupting contact between spinal motoneurons and muscle fibers. Axotomized motoneurons typically degenerate within two to three weeks after avulsion, the situation being exacerbated by an increased glial response and chronic inflammation. Nevertheless, root reimplantation has been observed to stimulate regenerative potential in some motoneurons, serving as a model for CNS/PNS regeneration. We hypothesized that a combination of neuroprotective and immunomodulatory therapies is capable of enhancing regenerative responses following nerve root injury and repair. A heterologous fibrin biopolymer (HFB) was used for surgical repair; dimethyl fumarate (DMF) was used for neuroprotection and immunomodulation; and adipose tissue-derived mesenchymal stem cells (AT-MSCs) were used as a source of trophic factors and cytokines that may further enhance neuronal survival. Thus, adult female Lewis rats underwent unilateral VRA of the L4–L6 roots, followed by reimplantation with HFB, AT-MSCs transplantation, and daily DMF treatment for four weeks, with a 12-week postoperative survival period. An evaluation of the results focused on light microscopy, qRT-PCR, and the Catwalk motor function recovery system. Data were analyzed using one-way or two-way ANOVA (p < 0.05). The results indicate that the combined therapy resulted in a reduced glial response and a 70% improvement in behavioral motor recovery. Overall, the data support the potential of combined regenerative approaches after spinal cord root injury.-
Descrição: dc.descriptionLaboratory of Nerve Regeneration University of Campinas (UNICAMP), SP-
Descrição: dc.descriptionCenter for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP), SP-
Descrição: dc.descriptionCenter for Translational Sciences and Biopharmaceuticals Development, SP-
Descrição: dc.descriptionMedical School São Paulo State University (UNESP), SP-
Descrição: dc.descriptionCenter for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP), SP-
Descrição: dc.descriptionMedical School São Paulo State University (UNESP), SP-
Idioma: dc.languageen-
Relação: dc.relationBiology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectgliosis-
Palavras-chave: dc.subjectinflammation-
Palavras-chave: dc.subjectspinal cord injury-
Palavras-chave: dc.subjectstem cell therapy-
Título: dc.titleCombination of Adult Mesenchymal Stem Cell Therapy and Immunomodulation with Dimethyl Fumarate Following Spinal Cord Ventral Root Repair-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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