Metallomic Approach to Mercury and Selenium in the Liver Tissue of Psectrogaster amazonica and Raphiodon vulpinus from the Brazilian Amazon

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorFederal University of Rondônia-
Autor(es): dc.contributorUniversity of Brasília (UNB)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorLouisiana State University Health Sciences Center (LSUHSC)-
Autor(es): dc.creatorBataglioli, Izabela-
Autor(es): dc.creatorVieira, José-
Autor(es): dc.creatorSiva, Joyce da-
Autor(es): dc.creatorAndrade, Luane-
Autor(es): dc.creatorFaria, Victor-
Autor(es): dc.creatorCorcoba, Rebeca-
Autor(es): dc.creatorAlmeida, Ronaldo de-
Autor(es): dc.creatorZara, Luiz-
Autor(es): dc.creatorBuzalaf, Marília-
Autor(es): dc.creatorAdamec, Jiri-
Autor(es): dc.creatorPadilha, Pedro-
Data de aceite: dc.date.accessioned2025-08-21T16:00:19Z-
Data de disponibilização: dc.date.available2025-08-21T16:00:19Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-10-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/ijms252211946-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/297214-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/297214-
Descrição: dc.descriptionThis paper reports the results of a mercury (Hg) and selenium (Se) metallomic study in the liver tissues of Psectrogaster amazonica and Raphiodon vulpinus from the Brazilian Amazon. Two-dimensional electrophoresis, graphite furnace atomic absorption spectrometry, and liquid chromatography-tandem mass spectrometry were performed. Hg and Se determinations allowed the calculation of Hg:Se and Se:Hg molar ratio and Se values for health benefits (Se HBVs). The Se:Hg values were >1 for both fish species, whereas the Se HBVs were >5 for P. amazonica and >10 for R. vulpinus, indicating that both possess Se reserves to control Hg toxicity. The metallomic data allowed the identification of 11 Hg/Se-associated protein spots in the two fish species, with concentrations in the range of 9.70 ± 0.14 and 28.44 ± 0.31 mg kg−1 of Hg and 16.15 ± 0.21 and 43.12 ± 0.51 mg kg−1 of Se. Five metal binding proteins (MBP) in the Hg/Se-associated protein spots in the liver proteome of P. amazonica and eight in R. vulpinus were identified, indicating the possible formation of Hg/Se complexes on the MBP structures. The activities analysis of catalase, superoxide dismutase, GPx enzymes, and lipoperoxide concentrations demonstrated that Hg-induced oxidative stress did not occur, possibly because both fish species possess Se reserves necessary to inhibit the Hg’s deleterious effects.-
Descrição: dc.descriptionSchool of Veterinary Medicine and Animal Science São Paulo State University (UNESP), SP-
Descrição: dc.descriptionInstitute of Biosciences São Paulo State University (UNESP), SP-
Descrição: dc.descriptionWolfgang C. Pfeiffer Environmental Biogeochemistry Laboratory Federal University of Rondônia, Porto Velho, RO-
Descrição: dc.descriptionCollege of Planaltina University of Brasília (UNB), DF-
Descrição: dc.descriptionDepartment of Biochemistry Bauru School of Dentistry University of São Paulo (USP), SP-
Descrição: dc.descriptionSchool of Medicine Louisiana State University Health Sciences Center (LSUHSC)-
Descrição: dc.descriptionSchool of Veterinary Medicine and Animal Science São Paulo State University (UNESP), SP-
Descrição: dc.descriptionInstitute of Biosciences São Paulo State University (UNESP), SP-
Idioma: dc.languageen-
Relação: dc.relationInternational Journal of Molecular Sciences-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectHg and Se in Amazon fish-
Palavras-chave: dc.subjectHg-induced oxidative stress-
Palavras-chave: dc.subjectmetal binding proteins-
Palavras-chave: dc.subjectmetalloproteomics-
Palavras-chave: dc.subjectSe/Hg-associated protein-
Título: dc.titleMetallomic Approach to Mercury and Selenium in the Liver Tissue of Psectrogaster amazonica and Raphiodon vulpinus from the Brazilian Amazon-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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