Moojecin: The first disintegrin from Bothrops moojeni venom and its antitumor activity in acute myeloid leukemia

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Federal de Uberlândia (UFU)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorAlmeida, Gabriela de Oliveira-
Autor(es): dc.creatorCintra, Adélia Cristina Oliveira-
Autor(es): dc.creatorSilva, Thiago Abrahão-
Autor(es): dc.creatorde Oliveira, Isadora Sousa-
Autor(es): dc.creatorCorreia, Lucas Ian Veloso-
Autor(es): dc.creatorTorquato, Ricardo José Soares-
Autor(es): dc.creatorFerreira Junior, Rui Seabra-
Autor(es): dc.creatorArantes, Eliane Candiani-
Autor(es): dc.creatorSampaio, Suely Vilela-
Data de aceite: dc.date.accessioned2025-08-21T20:31:38Z-
Data de disponibilização: dc.date.available2025-08-21T20:31:38Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-10-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.ijbiomac.2024.135066-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/297132-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/297132-
Descrição: dc.descriptionDisintegrins are a class of peptides found in snake venom that inhibit the activity of integrins, which are essential cell adhesion receptors in tumor progression and development. In this work, moojecin, a RGD disintegrin, was isolated from Bothrops moojeni snake venom, and its antitumor potential in acute myeloid leukemia (AML) HL-60 and THP-1 cells was characterized. The isolation was performed using a C18 reverse-phase column in two chromatographic steps, and its molecular mass is 7417.84 Da. N-terminal and de novo sequencing was performed to identify moojecin. Moojecin did not show cytotoxic or antiproliferative activity in THP-1 and HL-60 at tested concentrations, but it exhibited significant antimigratory activity in both cell lines, as well as inhibition of angiogenesis in the tube formation assay on Matrigel in a dose-dependent manner. A stronger interaction with integrin αVβ3 was shown in integrin interaction assays compared to α5β1, and the platelet aggregation assay indicated an IC50 of 5.039 μg/mL. Preliminary evaluation of disintegrin toxicity revealed no incidence of hemolysis or cytotoxic effects on peripheral blood mononuclear cells (PBMCs) across the tested concentrations. Thus, this is the first study to report the isolation, functional and structural characterization of a disintegrin from B. moojeni venom and bring a new perspective to assist in AML treatment.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionDepartment of Clinical Analysis Toxicology and Food Science School of Pharmaceutical Sciences of Ribeirão Preto University of São Paulo, SP-
Descrição: dc.descriptionDepartment of BioMolecular Sciences School of Pharmaceutical Sciences of Ribeirão Preto University of São Paulo, SP-
Descrição: dc.descriptionInstitute of Biotechnology Federal University of Uberlândia, MG-
Descrição: dc.descriptionDepartment of Biochemistry Federal University of São Paulo, SP-
Descrição: dc.descriptionCenter for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP), SP-
Descrição: dc.descriptionCenter for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP), SP-
Descrição: dc.descriptionFAPESP: 2021/11936-3-
Descrição: dc.descriptionFAPESP: 2022/04804-6-
Descrição: dc.descriptionFAPESP: 2022/05554-3-
Descrição: dc.descriptionCNPq: 303781/2022-0-
Descrição: dc.descriptionCNPq: 402352/2023-8-
Descrição: dc.descriptionCAPES: 88887.661488/2022-00-
Idioma: dc.languageen-
Relação: dc.relationInternational Journal of Biological Macromolecules-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAcute myeloid leukemia-
Palavras-chave: dc.subjectDisintegrins-
Palavras-chave: dc.subjectSnake venom-
Título: dc.titleMoojecin: The first disintegrin from Bothrops moojeni venom and its antitumor activity in acute myeloid leukemia-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.