Nitric Oxide Detection Using a Chemical Trap Method for Applications in Bacterial Systems †

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorCiência e Tecnologia Goiano-
Autor(es): dc.contributorCâmpus Henrique Santillo de Ciências Exatas e Tecnológicas Henrique Santillo-
Autor(es): dc.contributorSimple Agro Corporation-
Autor(es): dc.contributorCity University of New York-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorOliveira, Marilene Silva-
Autor(es): dc.creatorSantos, Karina F. D. N.-
Autor(es): dc.creatorde Paula, Railane Monteiro-
Autor(es): dc.creatorVitorino, Luciana C.-
Autor(es): dc.creatorBessa, Layara A.-
Autor(es): dc.creatorGreer, Alexander-
Autor(es): dc.creatorDi Mascio, Paolo-
Autor(es): dc.creatorde Souza, João C. P.-
Autor(es): dc.creatorMartin-Didonet, Claudia C. G.-
Data de aceite: dc.date.accessioned2025-08-21T16:12:53Z-
Data de disponibilização: dc.date.available2025-08-21T16:12:53Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-09-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/microorganisms11092210-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/297072-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/297072-
Descrição: dc.descriptionPlant growth-promoting bacteria (PGPB) can be incorporated in biofertilizer formulations, which promote plant growth in different ways, such as fixing nitrogen and producing phytohormones and nitric oxide (NO). NO is a free radical involved in the growth and defense responses of plants and bacteria. NO detection is vital for further investigation in different agronomically important bacteria. NO production in the presence of KNO3 was evaluated over 1–3 days using eight bacterial strains, quantified by the usual Griess reaction, and monitored by 2,3-diaminonaphthalene (DAN), yielding 2,3-naphthotriazole (NAT), as analyzed by fluorescence spectroscopy, gas chromatography–mass spectrometry, and high-performance liquid chromatography. The Greiss and trapping reaction results showed that Azospirillum brasilense (HM053 and FP2), Rhizobium tropici (Br322), and Gluconacetobacter diazotrophicus (Pal 5) produced the highest NO levels 24 h after inoculation, whereas Nitrospirillum amazonense (Y2) and Herbaspirillum seropedicae (SmR1) showed no NO production. In contrast to the literature, in NFbHP–NH4Cl–lactate culture medium with KNO3, NO trapping led to the recovery of a product with a molecular mass ion of 182 Da, namely, 1,2,3,4-naphthotetrazole (NTT), which contained one more nitrogen atom than the usual NAT product with 169 Da. This strategy allows monitoring and tracking NO production in potential biofertilizing bacteria, providing future opportunities to better understand the mechanisms of bacteria–plant interaction and also to manipulate the amount of NO that will sustain the PGPB.-
Descrição: dc.descriptionJohn Simon Guggenheim Memorial Foundation-
Descrição: dc.descriptionInstituto Federal de Educação Ciência e Tecnologia Goiano Departamento de Agroquímica, Campus Rio Verde, GO-
Descrição: dc.descriptionCâmpus Henrique Santillo de Ciências Exatas e Tecnológicas Henrique Santillo, BR 153 n° 3105—Fazenda Barreiro do Meio, GO-
Descrição: dc.descriptionSimple Agro Corporation, rua Parque General Borges Forte, 400, Jardim Goiás, GO-
Descrição: dc.descriptionDepartment of Chemistry Brooklyn College City University of New York-
Descrição: dc.descriptionThe Graduate Center City University of New York-
Descrição: dc.descriptionDepartamento de Bioquímica Instituto de Química Universidade de São Paulo, SP-
Descrição: dc.descriptionDepartamento de Química Faculdade de Ciências Universidade Estadual Paulista, Av. Eng. Luiz Edmundo Carrijo Coube, 14-01, SP-
Descrição: dc.descriptionDepartamento de Química Faculdade de Ciências Universidade Estadual Paulista, Av. Eng. Luiz Edmundo Carrijo Coube, 14-01, SP-
Idioma: dc.languageen-
Relação: dc.relationMicroorganisms-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectchromatography-
Palavras-chave: dc.subjectfluorescence-
Palavras-chave: dc.subjectGreiss reaction-
Palavras-chave: dc.subjectN2-fixing bacteria-
Palavras-chave: dc.subjectnitric oxide-
Título: dc.titleNitric Oxide Detection Using a Chemical Trap Method for Applications in Bacterial Systems †-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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