Interkingdom biofilm of Streptococcus pyogenes and Candida albicans: establishment of an in vitro model and dose-response validation of antimicrobials

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Autor(es): dc.contributorUniversity of Western São Paulo (UNOESTE)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Federal de Minas Gerais (UFMG)-
Autor(es): dc.creatorAlves, Guilherme dos Santos Gomes-
Autor(es): dc.creatorde Oliveira, Amanda Munarolo Piacenza-
Autor(es): dc.creatorRoseno, Ana Carolyna Becher-
Autor(es): dc.creatorRibeiro, Natália Pereira-
Autor(es): dc.creatorAlves, Maria do Socorro-
Autor(es): dc.creatorSampaio, Caio-
Autor(es): dc.creatordo Prado, Rosana Leal-
Autor(es): dc.creatorPessan, Juliano Pelim-
Autor(es): dc.creatorMonteiro, Douglas Roberto-
Data de aceite: dc.date.accessioned2025-08-21T16:06:16Z-
Data de disponibilização: dc.date.available2025-08-21T16:06:16Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1080/08927014.2024.2395390-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/297044-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/297044-
Descrição: dc.descriptionAlthough Streptococcus pyogenes and Candida albicans may colonize tonsillar tissues, the interaction between them in mixed biofilms has been poorly explored. This study established an interkingdom biofilm model of S. pyogenes and C. albicans and verified the dose-response validation of antimicrobials. Biofilms were formed on microplates, in the presence or absence of a conditioning layer of human saliva, using Brain Heart Infusion (BHI) broth or artificial saliva (AS) as a culture medium, and with variations in the microorganism inoculation sequence. Biofilms grown in AS showed higher mass than those grown in BHI broth, and an opposite trend was observed for metabolism. The number of S. pyogenes colonies was lower in AS. Amoxicillin and nystatin showed dose-dependent effects. The inoculation of the two species at the same time, without prior exposure to saliva, and using BHI broth would be the model of choice for future studies assessing the effects of antimicrobials on dual S. pyogenes/C. albicans biofilms.-
Descrição: dc.descriptionPostgraduate Program in Health Sciences University of Western São Paulo (UNOESTE)-
Descrição: dc.descriptionSchool of Dentistry University of Western São Paulo (UNOESTE)-
Descrição: dc.descriptionSchool of Dentistry Araçatuba Department of Preventive and Restorative Dentistry São Paulo State University (UNESP)-
Descrição: dc.descriptionPostgraduate Program in Animal Science University of Western São Paulo (UNOESTE)-
Descrição: dc.descriptionSchool of Dentistry Department of Community and Preventive Dentistry Universidade Federal de Minas Gerais (UFMG), MG-
Descrição: dc.descriptionSchool of Dentistry Araçatuba Department of Diagnosis and Surgery São Paulo State University (UNESP)-
Descrição: dc.descriptionSchool of Dentistry Araçatuba Department of Preventive and Restorative Dentistry São Paulo State University (UNESP)-
Descrição: dc.descriptionSchool of Dentistry Araçatuba Department of Diagnosis and Surgery São Paulo State University (UNESP)-
Formato: dc.format580-592-
Idioma: dc.languageen-
Relação: dc.relationBiofouling-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBiofilms-
Palavras-chave: dc.subjectCandida albicans-
Palavras-chave: dc.subjectStreptococcus pyogenes-
Palavras-chave: dc.subjecttonsillitis-
Título: dc.titleInterkingdom biofilm of Streptococcus pyogenes and Candida albicans: establishment of an in vitro model and dose-response validation of antimicrobials-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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