Identification of Proteomic Biomarkers in Proliferative Verrucous Leukoplakia through Liquid Chromatography With Tandem Mass Spectrometry

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorInstituto de los materiales de Santiago de Compostela (iMATUS)-
Autor(es): dc.contributorInstituto de Investigación Sanitaria de Santiago (IDIS) (ORALRES Group)-
Autor(es): dc.contributorInstituto de Investigación Sanitaria de Santiago (IDIS)-
Autor(es): dc.contributorHospital Clinico Universitario de Santiago-
Autor(es): dc.contributorUniversidade Federal Fluminense (UFF)-
Autor(es): dc.contributorNational Institute of Cancer/INCA-
Autor(es): dc.contributorUniversity of Turku-
Autor(es): dc.contributorUniversity of Helsinki-
Autor(es): dc.contributorHUSLAB-
Autor(es): dc.contributorUniversity of Oulu-
Autor(es): dc.contributorMedical Research Center Oulu-
Autor(es): dc.contributorFaculty of Physiotherapy-
Autor(es): dc.contributorSchool of Medicine-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorFederal University of Rio Grande do Sul (UFRGS)-
Autor(es): dc.creatorArroyo, Esteban-
Autor(es): dc.creatorPérez Sayáns, Mario-
Autor(es): dc.creatorLópez, Susana Bravo-
Autor(es): dc.creatorde Oliveira Barbeiro, Camila-
Autor(es): dc.creatorPalaçon, Mariana Paravani-
Autor(es): dc.creatorChamorro Petronacci, Cintia M.-
Autor(es): dc.creatorVence, María García-
Autor(es): dc.creatorVázquez, María del Pilar Chantada-
Autor(es): dc.creatorCarrión, Andrés Blanco-
Autor(es): dc.creatorSuárez Peñaranda, José M.-
Autor(es): dc.creatorGarcía, Abel García-
Autor(es): dc.creatorVila, Pilar Gándara-
Autor(es): dc.creatorAlmeida, Janete Días-
Autor(es): dc.creatorVeríssimo da Costa, Giovani Carlo-
Autor(es): dc.creatorSousa Nogueira, Fábio César-
Autor(es): dc.creatorMedeiros Evaristo, Joseph Albert-
Autor(es): dc.creatorde Abreu Pereira, Denise-
Autor(es): dc.creatorRintala, Mirjami-
Autor(es): dc.creatorSalo, Tuula-
Autor(es): dc.creatorRautava, Jaana-
Autor(es): dc.creatorIruegas, Elena Padín-
Autor(es): dc.creatorOliveira Alves, Monica G.-
Autor(es): dc.creatorFerrisse, Túlio Morandin-
Autor(es): dc.creatorAlbergoni da Silveira, Heitor-
Autor(es): dc.creatorLeón, Jorge Esquiche-
Autor(es): dc.creatorSilva, Evânio Vilela-
Autor(es): dc.creatorFlores, Isadora Luana-
Autor(es): dc.creatorBufalino, Andreia-
Data de aceite: dc.date.accessioned2025-08-21T19:42:28Z-
Data de disponibilização: dc.date.available2025-08-21T19:42:28Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-10-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.labinv.2023.100222-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/297038-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/297038-
Descrição: dc.descriptionProliferative verrucous leukoplakia (PVL) is an oral potentially malignant disorder associated with high risk of malignant transformation. Currently, there is no treatment available, and restrictive follow-up of patients is crucial for a better prognosis. Oral leukoplakia (OL) shares some clinical and microscopic features with PVL but exhibits different clinical manifestations and a lower rate of malignant transformation. This study aimed to investigate the proteomic profile of PVL in tissue and saliva samples to identify potential diagnostic biomarkers with therapeutic implications. Tissue and saliva samples obtained from patients with PVL were compared with those from patients with oral OL and controls. Label-free liquid chromatography with tandem mass spectrometry was employed, followed by qualitative and quantitative analyses, to identify differentially expressed proteins. Potential biomarkers were identified and further validated using immunohistochemistry. Staining intensity scan analyses were performed on tissue samples from patients with PVL, patients with OL, and controls from Brazil, Spain, and Finland. The study revealed differences in the immune system, cell cycle, DNA regulation, apoptosis pathways, and the whole proteome of PVL samples. In addition, liquid chromatography with tandem mass spectrometry analyses showed that calreticulin (CALR), receptor of activated protein C kinase 1 (RACK1), and 14-3-3 Tau-protein (YWHAQ) were highly expressed in PVL samples. Immunohistochemistry validation confirmed increased CARL expression in PVL compared with OL. Conversely, RACK1 and YWHA were highly expressed in oral potentially malignant disorder compared to the control group. Furthermore, significant differences in CALR and RACK1 expression were observed in the OL group when comparing samples with and without oral epithelial dysplasia, unlike the PVL. This research provides insights into the molecular mechanisms underlying these conditions and highlights potential targets for future diagnostic and therapeutic approaches.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery Araraquara School of Dentistry São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionOral Medicine Oral Surgery and Implantology Unit (MedOralRes) Faculty of Medicine and Dentistry University of Santiago de Compostela Instituto de los materiales de Santiago de Compostela (iMATUS)-
Descrição: dc.descriptionInstituto de Investigación Sanitaria de Santiago (IDIS) (ORALRES Group)-
Descrição: dc.descriptionProteomic Unit Instituto de Investigación Sanitaria de Santiago (IDIS)-
Descrição: dc.descriptionServicio de Anatomia Patológica Hospital Clinico Universitario de Santiago-
Descrição: dc.descriptionDepartment of Bioscience and Buccal Diagnosis São José dos Campos Science and Technologies Institute São Paulo State University (Unesp), São Paulo-
Descrição: dc.descriptionDepartment of Basic Sciences Nova Friburgo Health Institute Univ. Federal Fluminense Nova Friburgo Rio de Janeiro Brazil h Laboratory of Proteomics LADETEC Institute of Chemistry Federal University of Rio de Janeiro-
Descrição: dc.descriptionProgram on Cellular and Molecular Oncobiology Research Coordination National Institute of Cancer/INCA-
Descrição: dc.descriptionDepartment of Oral Pathology University of Turku-
Descrição: dc.descriptionDepartment of Oral and Maxillofacial Diseases Clinicum University of Helsinki-
Descrição: dc.descriptionDepartment of Pathology HUSLAB-
Descrição: dc.descriptionDepartment of Cancer and Translational Research Unit University of Oulu-
Descrição: dc.descriptionMedical Research Center Oulu-
Descrição: dc.descriptionHuman Anatomy and Embryology Area Faculty of Physiotherapy Department of Functional Biology and Health Sciences-
Descrição: dc.descriptionAnhembi Morumbi University School of Medicine, São Paulo-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics São Paulo State University (UNESP) School of Dentistry-
Descrição: dc.descriptionOral Pathology Department of Stomatology Public Oral Health and Forensic Dentistry Ribeirão Preto Dental School (FORP/USP) University of São Paulo, São Paulo-
Descrição: dc.descriptionOral Pathology Area Conservative Dentistry Department Dental School Federal University of Rio Grande do Sul (UFRGS)-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery Araraquara School of Dentistry São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionDepartment of Bioscience and Buccal Diagnosis São José dos Campos Science and Technologies Institute São Paulo State University (Unesp), São Paulo-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics São Paulo State University (UNESP) School of Dentistry-
Idioma: dc.languageen-
Relação: dc.relationLaboratory Investigation-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectcalreticulin-
Palavras-chave: dc.subjectleukoplakia-
Palavras-chave: dc.subjectproliferative verrucous leukoplakia-
Palavras-chave: dc.subjectproteomic-
Título: dc.titleIdentification of Proteomic Biomarkers in Proliferative Verrucous Leukoplakia through Liquid Chromatography With Tandem Mass Spectrometry-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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