Exploring the antifungal, antibiofilm and antienzymatic potential of Rottlerin in an in vitro and in vivo approach

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Autor(es): dc.contributorUniversidade Federal de Uberlândia (UFU)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorSilva, Nagela Bernadelli Sousa-
Autor(es): dc.creatorMenezes, Ralciane Paula-
Autor(es): dc.creatorGonçalves, Daniela Silva-
Autor(es): dc.creatorSantiago, Mariana Brentini-
Autor(es): dc.creatorConejo, Noemi Chagas-
Autor(es): dc.creatorSouza, Sara Lemes-
Autor(es): dc.creatorSantos, Anna Lívia Oliveira-
Autor(es): dc.creatorda Silva, Robinson Sabino-
Autor(es): dc.creatorRamos, Salvador Boccaletti-
Autor(es): dc.creatorFerro, Eloisa Amália Vieira-
Autor(es): dc.creatorMartins, Carlos Henrique Gomes-
Data de aceite: dc.date.accessioned2025-08-21T15:34:41Z-
Data de disponibilização: dc.date.available2025-08-21T15:34:41Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1038/s41598-024-61179-z-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/296994-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/296994-
Descrição: dc.descriptionCandida species have been responsible for a high number of invasive infections worldwide. In this sense, Rottlerin has demonstrated a wide range of pharmacological activities. Therefore, this study aimed to evaluate the antifungal, antibiofilm and antivirulence activity of Rottlerin in vitro against Candida spp. and its toxicity and antifungal activity in vivo. Rottlerin showed antifungal activity against all yeasts evaluated, presenting Minimum Inhibitory and Fungicidal Concentration (MIC and MFC) values of 7.81 to > 1000 µg/mL. Futhermore, it was able to significantly inhibit biofilm production, presenting Biofilm Inhibitory Concentration (MICB50) values that ranged from 15.62 to 250 µg/mL and inhibition of the cell viability of the biofilm by 50% (IC50) from 2.24 to 12.76 µg/mL. There was a considerable reduction in all hydrolytic enzymes evaluated, with emphasis on hemolysin where Rottlerin showed a reduction of up to 20%. In the scanning electron microscopy (SEM) analysis, Rottlerin was able to completely inhibit filamentation by C. albicans. Regarding in vivo tests, Rottlerin did not demonstrate toxicity at the therapeutic concentrations demonstrated here and was able to increase the survival of C. elegans larvae infected. The results herein presented are innovative and pioneering in terms of Rottlerin’s multipotentiality against these fungal infections.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)-
Descrição: dc.descriptionLaboratory of Antimicrobial Testing Institute of Biomedical Sciences (ICBIM) Federal University of Uberlândia (UFU), Av. Pará, 1720 - Umuarama-
Descrição: dc.descriptionTechnical School of Health (ESTES) Federal University of Uberlândia (UFU)-
Descrição: dc.descriptionInnovation Center in Salivary Diagnostic and Nanotheranostics Institute of Biomedical Sciences (ICBIM) Federal University of Uberlandia (UFU)-
Descrição: dc.descriptionDepartment of Engineering and Exact Sciences Faculty of Agricultural and Veterinary Sciences - Jaboticabal (FCAV) São Paulo State University (UNESP)-
Descrição: dc.descriptionLaboratory of Immunophysiology of Reproduction Institute of Biomedical Sciences (ICBIM) Federal University of Uberlandia-
Descrição: dc.descriptionDepartment of Engineering and Exact Sciences Faculty of Agricultural and Veterinary Sciences - Jaboticabal (FCAV) São Paulo State University (UNESP)-
Descrição: dc.descriptionFAPEMIG: 02067-22-
Descrição: dc.descriptionFAPEMIG: 12138-
Idioma: dc.languageen-
Relação: dc.relationScientific Reports-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAntibiofilm-
Palavras-chave: dc.subjectAntifungal activity-
Palavras-chave: dc.subjectCaenorhabditis elegans-
Palavras-chave: dc.subjectHydrolytic enzymes-
Palavras-chave: dc.subjectRottlerin-
Título: dc.titleExploring the antifungal, antibiofilm and antienzymatic potential of Rottlerin in an in vitro and in vivo approach-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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