A novel mannan-specific chimeric antigen receptor M-CAR redirects T cells to interact with Candida spp. hyphae and Rhizopus oryzae spores

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Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorFederal University of Alagoas-
Autor(es): dc.contributorFederal University of Mato Grosso do Sul-
Autor(es): dc.creatorGuimarães, Júlia Garcia-
Autor(es): dc.creatorde Campos, Gabriela Yamazaki-
Autor(es): dc.creatorMachado, Michele Procópio-
Autor(es): dc.creatorOliveira Brito, Patrícia Kellen Martins-
Autor(es): dc.creatordos Reis, Thaila Fernanda-
Autor(es): dc.creatorGoldman, Gustavo Henrique-
Autor(es): dc.creatorBonini Palma, Patricia Vianna-
Autor(es): dc.creatorde Campos Fraga-Silva, Thais Fernanda-
Autor(es): dc.creatorCavallin, Daniela Cardoso Umbelino-
Autor(es): dc.creatorVenturini, James-
Autor(es): dc.creatorda Silva, Thiago Aparecido-
Data de aceite: dc.date.accessioned2025-08-21T20:15:29Z-
Data de disponibilização: dc.date.available2025-08-21T20:15:29Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1080/21655979.2025.2458786-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/296992-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/296992-
Descrição: dc.descriptionInvasive fungal infections (IFIs) are responsible for elevated rates of morbidity and mortality, causing around of 1.5 million deaths annually worldwide. One of the main causative agents of IFIs is Candida albicans, and non-albicans Candida species have emerged as a spreading global public health concernment. Furthermore, COVID-19 has contributed to a boost in the incidence of IFIs, such as mucormycosis, in which Rhizopus oryzae is the most prevalent causative agent. The effector host immune response against IFIs depends on the activity of T cells, which are susceptible to the regulatory effects triggered by fungal virulence factors. The fungal cell wall plays a crucial role as a virulence factor, and its remodeling compromises the development of a specific T-cell response. The redirection of Jurkat T cells to target Candida spp. by recognizing targets expressed on the fungal cell wall can be facilitated using chimeric antigen receptor (CAR) technology. This study generated an M-CAR that contains an scFv with specificity to α-1,6 mannose backbone of fungal mannan, and the expression of M-CAR on the surface of modified Jurkat cells triggered a strong activation against Candida albicans (hyphae form), Candida tropicalis (hyphae form), Candida parapsilosis (pseudohyphal form), and Candida glabrata (yeast form). Moreover, M-CAR Jurkat cells recognized Rhizopus oryzae spores, which induced high expression of cell activation markers. Thus, a novel Mannan-specific CAR enabled strong signal transduction in modified Jurkat cells in the presence of Candida spp. or R. oryzae.-
Descrição: dc.descriptionDepartment of Cellular and Molecular Biology Ribeirao Preto Medical School University of São Paulo-
Descrição: dc.descriptionDepartment of Clinical Analysis School of Pharmaceutical Sciences in Araraquara Sao Paulo State University-
Descrição: dc.descriptionRibeirão Preto Pharmaceutical Sciences School University of São Paulo-
Descrição: dc.descriptionCenter for Cell-Based Therapy Regional Blood Center of Ribeirão Preto University of São Paulo-
Descrição: dc.descriptionInstitute of Biological and Health Sciences Federal University of Alagoas-
Descrição: dc.descriptionFaculty of Medicine Federal University of Mato Grosso do Sul-
Descrição: dc.descriptionDepartment of Clinical Analysis School of Pharmaceutical Sciences in Araraquara Sao Paulo State University-
Idioma: dc.languageen-
Relação: dc.relationBioengineered-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectcandida spp-
Palavras-chave: dc.subjectChimeric Antigen Receptor-
Palavras-chave: dc.subjectinvasive fungal infection-
Palavras-chave: dc.subjectjurkat cells-
Palavras-chave: dc.subjectmannan-
Palavras-chave: dc.subjectrhizopus oryzae-
Título: dc.titleA novel mannan-specific chimeric antigen receptor M-CAR redirects T cells to interact with Candida spp. hyphae and Rhizopus oryzae spores-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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