The Efficiency of Centrifugation in the Detection of Mollicutes in Bovine Milk

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorIndependent Researcher-
Autor(es): dc.creatorSalina, Anelise-
Autor(es): dc.creatorLopes, Bruna Churocof-
Autor(es): dc.creatorBarone, Fabiana Luccas-
Autor(es): dc.creatorLangoni, Helio-
Data de aceite: dc.date.accessioned2025-08-21T19:28:31Z-
Data de disponibilização: dc.date.available2025-08-21T19:28:31Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-09-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/ruminants3030021-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/296972-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/296972-
Descrição: dc.descriptionMastitis is one of the main diseases that affects dairy cattle. It is characterized by a multifactorial disease with multiple etiologies including microorganisms such as bacteria, viruses, fungi, yeasts, and algae. Mastitis caused by Mycoplasma spp. results in a contagious nature of infection and has been studied much in Brazil. The objectives of this research were (1) to investigate in clinical mastitis milk samples, by conventional PCR, the presence of the following mycoplasma species: Mycoplasma (M.) bovis, M. bovirhinis, M. bovigenitalium, M. californicum, and M. alkalescens and (2) compare the PCR Mollicutes detection rates using previous concentration and homogenization steps of the milk samples. Of the 993 clinical mastitis milk samples analyzed, 13.7% (136/993) and 8.0% (80/993) were positive for the Mollicutes class after concentration and homogenization, respectively, and all of them were PCR-negative for the five species investigated. Of the 70 expansion milk tanks analyzed, all of them were PCR-negative for the Mollicutes class. The importance of the previous concentration of milk in the routine of molecular analysis was evidenced when compared to the results obtained only with homogenization.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Animal Production and Preventive Veterinary Medicine School of Veterinary Medicine and Animal Science São Paulo State University-
Descrição: dc.descriptionIndependent Researcher-
Descrição: dc.descriptionDepartment of Animal Production and Preventive Veterinary Medicine School of Veterinary Medicine and Animal Science São Paulo State University-
Descrição: dc.descriptionFAPESP: 2018/09086-9-
Formato: dc.format233-239-
Idioma: dc.languageen-
Relação: dc.relationRuminants-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectclinical mastitis-
Palavras-chave: dc.subjectmammary mycoplasmosis-
Palavras-chave: dc.subjectMycoplasmaspp-
Palavras-chave: dc.subjectPCR-
Título: dc.titleThe Efficiency of Centrifugation in the Detection of Mollicutes in Bovine Milk-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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