Ethanolic extract of Casearia sylvestris and its clerodane diterpen (caseargrewiin F) protect against DNA damage at low concentrations and cause DNA damage at high concentrations in mice's blood cells

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorde Oliveira, Aline M.-
Autor(es): dc.creatorSantos, André Gonzaga dos-
Autor(es): dc.creatordos Santos, Raquel A.-
Autor(es): dc.creatorCsipak, Angelica R.-
Autor(es): dc.creatorOlivato, Camila-
Autor(es): dc.creatorda Silva, Isabel C.-
Autor(es): dc.creatorde Freitas, Maura B.-
Autor(es): dc.creatorBassi, Carmen L.-
Autor(es): dc.creatorCavalheiro, Alberto José-
Autor(es): dc.creatorBolzani, Vanderlan da Silva-
Autor(es): dc.creatorSilva, Dulce H. S.-
Autor(es): dc.creatorSakamoto-Hojo, Elza T.-
Autor(es): dc.creatorTakahashi, Catarina S.-
Autor(es): dc.creatorSoares, Christiane P.-
Data de aceite: dc.date.accessioned2021-03-10T17:56:46Z-
Data de disponibilização: dc.date.available2021-03-10T17:56:46Z-
Data de envio: dc.date.issued2014-05-20-
Data de envio: dc.date.issued2014-05-20-
Data de envio: dc.date.issued2009-11-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1093/mutage/gep034-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/26023-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/26023-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionBioprospecta-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento a Pesquisa-
Descrição: dc.descriptionProcesso FAPESP: 05/58472-9-
Descrição: dc.descriptionProcesso FAPESP: 03/02176-7-
Descrição: dc.descriptionBioprospecta: 2004/07932-7-
Descrição: dc.descriptionCNPq: 305615/2006-8-
Descrição: dc.descriptionCasearia sylvestris is used in Brazil as a popular medicine to treat ulcer, inflammation and tumour. Caseargrewiin F is a clerodane diterpene isolated from the ethanolic leaf extract of C.sylvestris. The aim of the study was to assess the capacity of the ethanolic extract of C.sylvestris leaves and caseargrewiin F to protect DNA and verify if both the compounds cause some DNA damage, using the micronucleus (MN) test and comet assay in mice. Balb-C mice were treated with the extract [3.13, 6.25, 12.5, 25, 50 and 75 mg/kg body weight (b.w.)] and caseargrewiin F (0.16, 0.32, 0.63, 1.3, 2.5 and 3.8 mg/kg b.w.) for 14 days. on day 15, DNA damage was induced by intra-peritoneal (i.p.) injection of cyclophosphamide (CP) (i.p.) at 50 mg/kg b.w. after the MN test and comet assay were performed. A protective effect of ethanolic extract was observed in MN test (6.25 and 12.5 mg/kg b.w.) and the comet assay (3.13 and 6.25, 12.5 and 25 mg/kg b.w.). Caseargrewiin F showed protective effect at 0.63, 1.3 and 2.5 mg/kg b.w. only in comet assay. We also tested the ability of compounds of C.sylvestris to induce MN and to increase the comet assay tail moment. The experimental design was similar to the DNA protection assay except that in test groups we omitted the CP challenge. We observed increased damage at 50 and 75 mg/kg b.w. of ethanolic extract of C.sylvestris and caseargrewiin F at 3.18 mg/kg b.w. in both the MN test and comet assay. We conclude that ethanolic extract of C. sylvestris and caseargrewiin F can protect cells against DNA damage induced by CP at low concentrations, but at high concentrations these compounds also induce DNA damage.-
Formato: dc.format501-506-
Idioma: dc.languageen-
Publicador: dc.publisherOxford University Press-
Relação: dc.relationMutagenesis-
Relação: dc.relation2.840-
Relação: dc.relation0,916-
Direitos: dc.rightsclosedAccess-
Título: dc.titleEthanolic extract of Casearia sylvestris and its clerodane diterpen (caseargrewiin F) protect against DNA damage at low concentrations and cause DNA damage at high concentrations in mice's blood cells-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.