Protein profiles identified by LC-MS/MS demonstrate change in beta oxidation, ketogenesis, and propionate metabolism in rumen epithelium with different additives

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorDSM Nutritional Products SA-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversity of Nebraska (UNL)-
Autor(es): dc.creatorRocha, Leone Campos-
Autor(es): dc.creatorAssunção, Andrey Sávio de Almeida-
Autor(es): dc.creatorMartins, Renata Aparecida-
Autor(es): dc.creatorCarvalho, Victor Valério de-
Autor(es): dc.creatorPerdigão, Alexandre-
Autor(es): dc.creatorBuzalaf, Marília Afonso Rabelo-
Autor(es): dc.creatorAdamec, Jiri-
Autor(es): dc.creatorBraga, Camila Pereira-
Autor(es): dc.creatorMillen, Danilo Domingues-
Autor(es): dc.creatorVieira, José Cavalcante Souza-
Autor(es): dc.creatorPadilha, Pedro de Magalhães-
Data de aceite: dc.date.accessioned2025-08-21T21:51:37Z-
Data de disponibilização: dc.date.available2025-08-21T21:51:37Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-08-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.livsci.2023.105269-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/250039-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/250039-
Descrição: dc.descriptionReplacement of ionophore feed additives by essential oils demonstrates differences in energy metabolism. The mechanism of action of essential oils depends on the ruminal pH, therefore, feedlot diets with high starch inclusions tend to show greater effectiveness of these natural additives. The aim of this study is to map the proteome of the ruminal epithelium of feedlot Nellore cattle (n = 60) with different additives (monensin, blend of essential oils + exogenous α-amylase) and varying levels of starch (25% and 45%) Two-dimensional polyacrylamide gel electrophoresis was used to separate the proteome of ruminal epithelium. The differentially expressed protein spots were characterized by isoelectric point, molecular mass, volume, and intensity of the spots and identified by liquid chromatography tandem mass spectrometry (LC-MS/MS). Diets using an essential oil blend associated with exogenous amylase promoted greater expression of macromolecules from the degradation of carbohydrates through the glycolytic and ketogenesis pathway. Fourteen proteins were identified in upregulation and the presence of proteins involved in glucose oxidation as well as Hydroxymethylglutaryl–CoA lyase (HMGCL), which catalyzes part of the metabolic intermediate metabolism—a key step in ketogenesis. Our results suggest that there was an increase in glycolysis from the oxidation of Glyceraldehyde-3-phosphate dehydrogenase (GAPDH GAPD), which participates in the first step of acetate and butyrate production and the oxidative decarboxylation of the ruminal epithelium of feedlot Nellore cattle. Monensin increased precursors of propionate, such as methylmalonyl-CoA mutase (MUT), which suggests greater synthesis of propionate via propionyl-CoA upon entry into the citric acid cycle as succinyl-CoA, which can increase feed efficiency and reduces feed intake.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Veterinary Medicine and Animal Science, São Paulo-
Descrição: dc.descriptionDSM Nutritional Products SA-
Descrição: dc.descriptionUniversity of São Paulo (USP) Bauru School of Dentistry, São Paulo-
Descrição: dc.descriptionUniversity of Nebraska (UNL) Department of Biochemistry-
Descrição: dc.descriptionSão Paulo State University UNESP College of Technology and Agricultural Sciences, São Paulo-
Descrição: dc.descriptionSão Paulo State University (UNESP) Institute of Biosciences, São Paulo-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Veterinary Medicine and Animal Science, São Paulo-
Descrição: dc.descriptionSão Paulo State University UNESP College of Technology and Agricultural Sciences, São Paulo-
Descrição: dc.descriptionSão Paulo State University (UNESP) Institute of Biosciences, São Paulo-
Descrição: dc.descriptionCAPES: 001-
Descrição: dc.descriptionCAPES: 88881.3107432018–0.1-
Descrição: dc.descriptionFAPESP: FAPESP 19/02538–4-
Idioma: dc.languageen-
Relação: dc.relationLivestock Science-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectEnergy metabolism-
Palavras-chave: dc.subjectMass spectrometry-
Palavras-chave: dc.subjectMonensin-
Palavras-chave: dc.subjectPropionate-
Título: dc.titleProtein profiles identified by LC-MS/MS demonstrate change in beta oxidation, ketogenesis, and propionate metabolism in rumen epithelium with different additives-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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