Zerumbone Disturbs the Extracellular Matrix of Fluconazole-Resistant Candida albicans Biofilms

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorAbreu-Pereira, César Augusto-
Autor(es): dc.creatorGorayb-Pereira, Ana Luiza-
Autor(es): dc.creatorMenezes Noveletto, João Vinícius-
Autor(es): dc.creatorJordão, Cláudia Carolina-
Autor(es): dc.creatorPavarina, Ana Cláudia-
Data de aceite: dc.date.accessioned2025-08-21T20:58:13Z-
Data de disponibilização: dc.date.available2025-08-21T20:58:13Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-05-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/jof9050576-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/250009-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/250009-
Descrição: dc.descriptionThis study assessed the effect of zerumbone (ZER) against fluconazole-resistant (CaR) and -susceptible Candida albicans (CaS) biofilms and verified the influence of ZER on extracellular matrix components. Initially, to determine the treatment conditions, the minimum inhibitory concentration (MIC), the minimum fungicidal concentration (MFC) and the survival curve were evaluated. Biofilms were formed for 48 h and exposed to ZER at concentrations of 128 and 256 µg/mL for 5, 10 and 20 min (n = 12). One group of biofilms did not receive the treatment in order to monitor the effects. The biofilms were evaluated to determine the microbial population (CFU/mL), and the extracellular matrix components (water-soluble polysaccharides (WSP), alkali-soluble polysaccharides (ASPs), proteins and extracellular DNA (eDNA), as well as the biomass (total and insoluble) were quantified. The MIC value of ZER for CaS was 256 μg/mL, and for CaR, it was 64 μg/mL. The survival curve and the MFC value coincided for CaS (256 μg/mL) and CaR (128 μg/mL). ZER reduced the cellular viability by 38.51% for CaS and by 36.99% for CaR. ZER at 256 µg/mL also reduced the total biomass (57%), insoluble biomass (45%), WSP (65%), proteins (18%) and eDNA (78%) of CaS biofilms. In addition, a reduction in insoluble biomass (13%), proteins (18%), WSP (65%), ASP (10%) and eDNA (23%) was also observed in the CaR biofilms. ZER was effective against fluconazole-resistant and -susceptible C. albicans biofilms and disturbed the extracellular matrix.-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)-
Idioma: dc.languageen-
Relação: dc.relationJournal of Fungi-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectbiofilms-
Palavras-chave: dc.subjectCandida albicans-
Palavras-chave: dc.subjectphytotherapy-
Palavras-chave: dc.subjectzerumbone-
Título: dc.titleZerumbone Disturbs the Extracellular Matrix of Fluconazole-Resistant Candida albicans Biofilms-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.