Aerobiology of the Wheat Blast Pathogen: Inoculum Monitoring and Detection of Fungicide Resistance Alleles

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorNIAB-
Autor(es): dc.contributorRothamsted Research-
Autor(es): dc.contributorIDR–Paraná/IAPAR-
Autor(es): dc.contributorUniversidad Agraria del Ecuador (UAE)-
Autor(es): dc.contributorUniversidade Federal de Sergipe (UFS)-
Autor(es): dc.contributorTechnical University of Manabí-
Autor(es): dc.contributorWageningen University & Research-
Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.creatorVicentini, Samara Nunes Campos-
Autor(es): dc.creatorHawkins, Nichola J.-
Autor(es): dc.creatorKing, Kevin M.-
Autor(es): dc.creatorMoreira, Silvino Intra-
Autor(es): dc.creatorde Paiva Custódio, Adriano Augusto-
Autor(es): dc.creatorLeite Júnior, Rui Pereira-
Autor(es): dc.creatorPortalanza, Diego-
Autor(es): dc.creatorGarcés-Fiallos, Felipe Rafael-
Autor(es): dc.creatorKrug, Loane Dantas-
Autor(es): dc.creatorWest, Jonathan S.-
Autor(es): dc.creatorFraaije, Bart A.-
Autor(es): dc.creatorDe Jesus Júnior, Waldir Cintra-
Autor(es): dc.creatorCeresini, Paulo Cezar-
Data de aceite: dc.date.accessioned2025-08-21T19:40:56Z-
Data de disponibilização: dc.date.available2025-08-21T19:40:56Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-05-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/agronomy13051238-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/249999-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/249999-
Descrição: dc.descriptionWheat blast, caused by the ascomycetous fungus Pyricularia oryzae Triticum lineage (PoTl), is mainly controlled by fungicide use, but resistance to the main fungicide groups—sterol demethylase (DMI), quinone outside (QoI), and succinate dehydrogenase inhibitors (SDHI)—has been reported in Brazil. In order to rationalize fungicide inputs (e.g., choice, timing, dose-rate, spray number, and mixing/alternation) for managing wheat blast, we describe a new monitoring tool, enabling the quantitative measurement of pathogen’s inoculum levels and detection of fungicide resistance alleles. Wheat blast airborne spores (aerosol populations) were monitored at Londrina in Paraná State, a major wheat cropping region in Brazil, using an automated high-volume cyclone coupled with a lab-based quantitative real-time PCR (qPCR) assay. The objectives of our study were as follows: (1) to monitor the amount of PoTl airborne conidia during 2019–2021 based on DNA detection, (2) to reveal the prevalence of QoI resistant (QoI-R) cytochrome b alleles in aerosol populations of wheat blast, and (3) to determine the impact of weather on the dynamics of wheat blast aerosol populations and spread of QoI resistant alleles. PoTl inoculum was consistently detected in aerosols during the wheat cropping seasons from 2019 to 2021, but amounts varied significantly between seasons, with highest amounts detected in 2019. High peaks of PoTl DNA were also continuously detected during the off-season in 2020 and 2021. The prevalence of QoI resistant (QoI-R) cytochrome b G143A alleles in aerosol populations was also determined for a subset of 10 PoTl positive DNA samples with frequencies varying between 10 and 91% using a combination of PCR-amplification and SNP detection pyrosequencing. Statistically significant but low correlations were found between the levels of pathogen and the weather variables. In conclusion, for wheat blast, this system provided prior detection of airborne spore levels of the pathogen and of the prevalence of fungicide resistance alleles.-
Descrição: dc.descriptionRothamsted Research-
Descrição: dc.descriptionDepartment of Crop Protection Agricultural Engineering and Soil Sao Paulo State University UNESP, SP-
Descrição: dc.descriptionNational Institute of Agricultural Botany NIAB-
Descrição: dc.descriptionProtecting Crops and Environment Rothamsted Research-
Descrição: dc.descriptionParaná Agricultural Development Institute IDR–Paraná/IAPAR, PR-
Descrição: dc.descriptionCarrera de Ingeniería Ambiental Facultad de Ciencias Agrarias Instituto de Investigación “Ing. Jacobo Bucaram Ortiz Ph.D” Universidad Agraria del Ecuador (UAE), Avenida 25 de Julio, Guayas-
Descrição: dc.descriptionCenter of Natural and Exact Sciences Department of Physics Federal University of Santa Maria (UFSM), Avenida Roraima 1000, RS-
Descrição: dc.descriptionLaboratory of Phytopathology Faculty of Agronomic Engineering Technical University of Manabí, Experimental Campus La Teodomira-
Descrição: dc.descriptionBU Biointeractions & Plant Health Wageningen Plant Research Wageningen University & Research-
Descrição: dc.descriptionCenter of Natural Science Federal University of São Carlos UFScar, Lagoa do Sino Campus, SP-
Descrição: dc.descriptionDepartment of Crop Protection Agricultural Engineering and Soil Sao Paulo State University UNESP, SP-
Idioma: dc.languageen-
Relação: dc.relationAgronomy-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectairborne spores-
Palavras-chave: dc.subjectepidemic predictors-
Palavras-chave: dc.subjectintegrated disease management-
Palavras-chave: dc.subjectPyricularia oryzae Triticum lineage-
Título: dc.titleAerobiology of the Wheat Blast Pathogen: Inoculum Monitoring and Detection of Fungicide Resistance Alleles-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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